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Showing: 1181 - 1200 of 23793 results

  1. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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  2. pdCas9-M-3A2

    Plasmid
    #73435
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3A2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73435-plasmid-map-sequence-id-135340

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  3. pdCas9-M-4A6

    Plasmid
    #73434
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 4A6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73434-plasmid-map-sequence-id-135334

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  4. pdCas9-M-C4

    Plasmid
    #73427
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73427-plasmid-map-sequence-id-135327

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  5. pdCas9-M-3F2

    Plasmid
    #73428
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73428-plasmid-map-sequence-id-135328

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  6. pdCas9-M-4F2

    Plasmid
    #73431
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 4F2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73431-plasmid-map-sequence-id-135331

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  7. pdCas9-M-1B6

    Plasmid
    #73430
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1B6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73430-plasmid-map-sequence-id-139104

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  8. pdCas9-M-3H5

    Plasmid
    #73429
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3H5 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73429-plasmid-map-sequence-id-135329

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  9. pdCas9-M-1D4

    Plasmid
    #73433
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1D4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73433-plasmid-map-sequence-id-135333

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  10. pdCas9-M-1E4

    Plasmid
    #73436
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1E4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73436-plasmid-map-sequence-id-135335

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  11. pdCas9-M-5F5

    Plasmid
    #73432
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 5F5 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73432-plasmid-map-sequence-id-135332

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  12. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

    Plasmid
    #110547
    Purpose
    CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.
    Depositor
    Ichiro Matsumura
    Insert
    dCas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pBAD
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    110547-plasmid-map-sequence-id-210556

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  14. pZ8-T_dCas9

    Plasmid
    #74062
    Purpose
    pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74062-plasmid-map-sequence-id-137972

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  15. pAL-pyk_NT

    Plasmid
    #74072
    Purpose
    pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pyk (NT)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74072-plasmid-map-sequence-id-314169

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  16. pAL-pgi_T

    Plasmid
    #74066
    Purpose
    pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pgi (T)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pAL-pck_T

    Plasmid
    #74068
    Purpose
    pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pck (T)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pAL-pck_NT

    Plasmid
    #74069
    Purpose
    pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pck (NT)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pAL-pgi_NT

    Plasmid
    #74067
    Purpose
    pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pgi (NT)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74067-plasmid-map-sequence-id-405024

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Showing: 1181 - 1200 of 23793 results