We narrowed to 8,785 results for: sgrna

1. 7a sgRNA for EJ7-GFP and 4-μHOM reporters

   Plasmid

   #113620

   Purpose

   sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7a sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pCAG-CFP-SaCas9-HF (without sgRNA)

   Plasmid

   #134470

   Purpose

   Human expression vector for SaCas9-HF variant.

   Depositor

   Zongli Zheng

   Article

   Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

   Insert

   Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
   

   Use

   CRISPR

   Tags

   SV40NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   R245A, N413A, N419A, R654A

   Promoter

   CAG

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

   Plasmid

   #71485

   Purpose

   Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-SasgRNA scaffold-CMV-nSaCas9

   Plasmid

   #171695

   Purpose

   Expresses nSaCas9 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   sgRNA scaffold-nSaCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   U6 and CMV

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGW03 AAV-TBG-Cre-sgRNA

   Plasmid

   #192163

   Purpose

   AAV-TBG-Cre-sgRNA

   Depositor

   Sidi Chen

   Article

   Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPUR-hU6-sgRNA-Sirius-8XMS2

   Plasmid

   #121942

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-8XMS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

   Plasmid

   #194284

   Purpose

   EF1a-dCas9-KRAB-GFP with nontarget sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

   Plasmid

   #102798

   Purpose

   Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Inserts

   sgRNA
   EGFP
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   H231L

   Promoter

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL-U6-sgRNA-SFFV-Puro-P2A-EGFP

   Plasmid

   #175037

   Purpose

   Lentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter.

   Depositor

   Kristian Helin

   Article

   Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

   Insert

   Sp sgRNA scaffold
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-U6-sgRNA(CTRL)-CMV-eGFP

    Plasmid

    #194017

    Purpose

    Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(CTRL)
    eGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. peSpCas9(1.1)-2×sgRNA (empty, donor)

    Plasmid

    #80768

    Purpose

    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSK-mU6-Cj-sgRNA-SV40-puro

    Plasmid

    #192128

    Purpose

    Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Cj-SgRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPUR-mU6-sgRNA-Sirius-8XPP7

    Plasmid

    #121943

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid

    #181792

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

    Plasmid

    #172436

    Purpose

    Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    Cre
    mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLH-sgRNA-Sirius-4X(MS2-PP7)

    Plasmid

    #121941

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-4X(MS2-PP7)
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere

    Plasmid

    #85715

    Purpose

    Telomere-targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid

    #194715

    Purpose

    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sites

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid

    #195741

    Purpose

    Base editing for making a pre-stop codon in mmoX using phenol inducible system

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Po (phoenol-inducible promoter)

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

    Plasmid

    #164471

    Purpose

    Expresses sgRNA targeting human GJA1 locus

    Depositor

    Markus Kuehn

    Article

    Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

    Insert

    Gap junction alpha-1 (GJA1) (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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