161,568 results

1. pANCH2-Nat

   Plasmid

   #87249

   Purpose

   gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   Nat
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-yomEos2-Kan

   Plasmid

   #44944

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomEos2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. R26TV CAG LSL 2-5

   Plasmid

   #68412

   Purpose

   Rosa26 targeting vector backbone for use with GMAP

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Type

   Empty backbone

   Use

   Cre/Lox and Mouse Targeting; Gmap

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4046768

   Plasmid

   #145763

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pUC19_CoV-2_linker

   Plasmid

   #211731

   Purpose

   Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

   Depositor

   Thomas Klimkait

   Article

   Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

   Insert

   Linker fragment SARS-CoV-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQTEV-TAGLN2

   Plasmid

   #31569

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   transgelin 2 (TAGLN2 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_HCK-1/1

   Plasmid

   #91273

   Purpose

   Protein expression and purification of human SH3 domain construct HCK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   HCK-1/1 (HCK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAMKIIa-TetOn3G

   Plasmid

   #133727

   Purpose

   Plasmid contains the TetOn3G-transactivator sequence under control of a Camk2a promoter.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   TetOn3G
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCamk2a

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p663-UBC-TurboID-V5-BRSK2_IDG-K

   Plasmid

   #135272

   Purpose

   Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   TurboID-V5-BRSK2 (BRSK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-rat PKA-RIIbeta-mEGFP

    Plasmid

    #45523

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RII beta tagged by mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMB413a

    Plasmid

    #20058

    Depositor

    Stephen Sligar

    Article

    Springer et al Proc Natl Acad Sci U S A. 1987 Dec . 84(

    Insert

    Sperm Whale Myoglobin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

12. HTR1E-Tango

    Plasmid

    #66407

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR1E (HTR1E Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pABY-c15

    Plasmid

    #201768

    Purpose

    Expresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    NbALFA
    

    Use

    Tags

    40aa Linker, mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWPI-mPAX4

    Plasmid

    #114306

    Purpose

    Constitutive expression of the Pax4 trancription factor

    Depositor

    Patrick Salmon

    Insert

    PAX4 (Pax4 Mouse)
    

    Use

    Lentiviral

    Tags

    c-myc epitope, 6xHis tag

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

    Plasmid

    #164041

    Purpose

    CRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B gene

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    mCherry-CRISPR-TagV2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbA8a-RFP

    Plasmid

    #35271

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF2304

    Plasmid

    #142020

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CIITA (CIITA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-mCherry-P2A-strepII-PPPCB

    Plasmid

    #67923

    Purpose

    Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. ZJOM30

    Plasmid

    #133673

    Purpose

    Integration of ERG6-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on ER and lipid droplets.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ERG6-GFP (ERG6 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-EGFP-hChibby1

    Plasmid

    #89770

    Purpose

    Labels mother centrioles/basal bodies

    Depositor

    Ken-Ichi Takemaru

    Insert

    Chibby1 (CBY1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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