We narrowed to 4,921 results for: gne

1. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

   Plasmid

   #61966

   Purpose

   Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   N-mCherry-ME(pS)VD
   TRAP(RK)-C-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD and PLtetO

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7C-HSPA1L

   Plasmid

   #177660

   Purpose

   Recombinant protein synthesis of human HSPA1L

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSPA1L (HSPA1L Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYZ164

   Plasmid

   #98408

   Purpose

   Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Insert

   gRNA targeting Sp.his3 (his3 Fission Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE:CloverCP-EFS:ZeoR

   Plasmid

   #68478

   Purpose

   Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   CloverCP
   Zeocin Resistance
   

   Use

   Lentiviral

   Tags

   "CP" degradation domain

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and pTRE

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. dFP-mini

   Plasmid

   #120543

   Purpose

   Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

   Depositor

   Brian Chow

   Article

   Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

   Insert

   de novo fluorescence-activating protein mini (dFP-mini)
   

   Use

   Tags

   6xHis - TEV cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDS277

   Plasmid

   #34973

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   V529N

   Promoter

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK_ToeRepG2_N02_switch

   Plasmid

   #132742

   Purpose

   T7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporter

   Depositor

   Peng Yin, Alexander Green

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   ToeRepG2_N02_switch
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5/FRT-ChemoL-ATP

   Plasmid

   #193831

   Purpose

   CMV overexpression of ChemoL-ATP in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoL-ATP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW2-Smed-Slit-1

   Plasmid

   #182264

   Purpose

   dsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1

   Depositor

   Daniel Lobo

   Article

   Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

   Insert

   Smed-Slit-1
   

   Use

   Gibson cloning vector for synthesis of single and…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDB364

    Plasmid

    #110275

    Purpose

    N-terminal 10-His DsbC TEV-peptide fusion

    Depositor

    Christopher Bahl

    Article

    Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    10-His, TEV cleavage peptide, and mature DsbC (i.…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK_ToeRepG2_N19_trigger

    Plasmid

    #132747

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNA

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N19_trigger
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TOPO pTRE

    Plasmid

    #68457

    Purpose

    TOPO construct expressing pTRE for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Tetracycline Response Element Promoter
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRE:shRNA A-pGK:rtTA-Cre

    Plasmid

    #68471

    Purpose

    Inducible lentivirus expressing an arbitrary shRNA and Cre

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    GFP-shRNA A-miR30
    rtTA-2A-Cre
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK and pTRE

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Twist-siRNA7

    Plasmid

    #8457

    Depositor

    Bob Weinberg

    Article

    Yang et al Cell 2004 Jun 25;117(7):927-39.

    Insert

    siTwist (Twist1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pET15b-mLin28A-cHIS

    Plasmid

    #137998

    Purpose

    Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cells

    Depositor

    Judy Lieberman

    Article

    O'Day et al J Biol Chem. 2015 Jul 17;290(29):17909-2

    Insert

    mouse Lin28A, CSD and ZnFs (residues 36–186)
    

    Use

    Tags

    C-terminal His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV Puro-UBC:GFP

    Plasmid

    #68482

    Purpose

    Retrovirus expressing GFP driven by UBC promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Puromycin Resistance
    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and UBC

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. TOPO rtTA3-2A-Cre

    Plasmid

    #68448

    Purpose

    TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    rtTA3-2A-Cre
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in mice

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Twist-siRNA5

    Plasmid

    #8456

    Depositor

    Bob Weinberg

    Article

    Yang et al Cell 2004 Jun 25;117(7):927-39.

    Insert

    siTwist (Twist1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pET-51b(+) ChemoG 3.2

    Plasmid

    #193803

    Purpose

    Protein production of ChemoG3.2 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG 3.2
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETCON-TNF

    Plasmid

    #67748

    Purpose

    Tumor Necrosis Factor Alpha Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    TNF-Alpha
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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