We narrowed to 2,921 results for: human cdnas

1. pcDNA5FRT/TO-VCPIP1-Strep-HA

   Plasmid

   #113482

   Purpose

   Expression of human VCPIP1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   VCPIP1 (VCPIP1 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-β2AR-μDpTT

   Plasmid

   #182326

   Purpose

   Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   beta2AR-μDronpa-TagRFP-T (ADRB2 Human)
   

   Use

   Tags

   Full-length human beta2 adrenergic receptor

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-RIIα-μDpTT

   Plasmid

   #182327

   Purpose

   PKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   RIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
   

   Use

   Tags

   Full-length human PKA type 2alpha regulatory subu…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-4LNDDE488Q-pCDNA3.1(+)

   Plasmid

   #172314

   Purpose

   Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)

   Depositor

   Joshua Rosenthal

   Article

   Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain E488Q
   

   Use

   Tags

   6xHis, Flag, and Nuclear Localization Signal of S…

   Expression

   Mammalian

   Mutation

   changed the Glutamic acid 488 in the Deaminase Do…

   Promoter

   CMV

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-PKHD1-myc

   Plasmid

   #108407

   Purpose

   Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

   Depositor

   Gregory Germino

   Article

   Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

   Insert

   PKHD1 (PKHD1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.2-GJA1-20K-V5

   Plasmid

   #49859

   Purpose

   Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   GJA1-20k (GJA1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   GJA1-20k fragment corresponds to residues 213-382…

   Promoter

   CMV

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5-GFP-PKHD1

   Plasmid

   #108408

   Purpose

   Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

   Depositor

   Gregory Germino

   Article

   Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

   Insert

   PKHD1 (PKHD1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-SARS-1-RBD-8h

   Plasmid

   #161821

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T320-D518)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.1 hSCN5A-L325R

   Plasmid

   #145373

   Purpose

   expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

   Depositor

   Hugues Abriel

   Article

   Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

   Insert

   sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Leucine 325 to Arginine

   Promoter

   CMV

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-V5-PTPN14-del-N

    Plasmid

    #61004

    Purpose

    Expresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 delete N (PTPN14 Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 17-310

    Promoter

    CMV

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3-V5-PTPN14-del-C

    Plasmid

    #61005

    Purpose

    Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 delete C (PTPN14 Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 934-1187

    Promoter

    CMV

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

12. FLAG-HA-FbxO10-pcDNA3.1-

    Plasmid

    #52508

    Purpose

    expresses human FbxO10 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO10 (FBXO10 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_Hygro(+)_GRHL1-L3F6H

    Plasmid

    #177765

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-INSRR-V5/HIS

    Plasmid

    #201981

    Purpose

    expression of human INSRR receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    INSRR receptor tyrosine kinase (INSRR Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA 3.1-TSF-ε

    Plasmid

    #196583

    Purpose

    Expresses the two Strep/one FLAG (TSF)-tagged human AP-4 ε subunit in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    AP-4 ε subunit (AP4E1 Human)
    

    Use

    Tags

    two Strep/one FLAG (TSF)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA GSK3 beta S9A pcDNA3

    Plasmid

    #14754

    Purpose

    Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

    Depositor

    Jim Woodgett

    Article

    Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

    Insert

    GSK3 beta S9A (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S9A. Constitutively active mutant.

    Promoter

    Available since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA5-eYFP-FKBP-MAD1-WT

    Plasmid

    #114037

    Purpose

    Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

    Depositor

    Geert Kops

    Article

    Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

    Insert

    Human MAD1 (MAD1L1 Human)
    

    Use

    Tags

    eYFP-FKBP12

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5 FRT TO GFP hBub1 WT

    Plasmid

    #59812

    Purpose

    Allows the integration of GFP hBub1 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

    Insert

    Bub1 (BUB1 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA6-LRRC55-V5-Flag-His

    Plasmid

    #127819

    Purpose

    Expression of human LRRC55 in mammalian cells

    Depositor

    Jiusheng Yan

    Article

    Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

    Insert

    LRRC55 (LRRC55 Human)
    

    Use

    Tags

    V5, FLAG, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-Flag-H2A K13R

    Plasmid

    #63566

    Purpose

    Expression of human H2A with K13R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine at 13 is now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only