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GFP-C1-CAMKIIalpha-T286A

Plasmid

#21220

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CAMKIIalpha-T286A (Camk2a Rat)

Tags

GFP

Expression

Mammalian

Mutation

T286A (Lab plasmid KS 0003).

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2

Plasmid

#44104

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
Punc-4::NGL-1::spGFP1-10

Plasmid

#65827

Purpose

For trans-synaptic labeling

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

NLG-1 (nlg-1 Nematode)

Tags

spGFP1-10

Expression

Worm

Promoter

Punc-4

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIalpha-K42R

Plasmid

#21221

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CAMKIIalpha-K42R (Camk2a Rat)

Tags

GFP

Expression

Mammalian

Mutation

K42R (Lab plasmid KS 0007).

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
FusionRed-Lifeact-7

Plasmid

#54778

Purpose

Localization: Actin, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Lifeact-FusionRed

Expression

Mammalian

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCMV/MASC_(GLuc)_TOP1

Plasmid

#68439

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBbE5a-Opto-Cre-Vvd

Plasmid

#134404

Purpose

Light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

Opto-Cre-Vvd

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ccdb-DddA(G1397C)-UGI-Puro

Plasmid

#173230

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro

Plasmid

#173231

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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P023-mPB-synHEK3-BsaI

Plasmid

#239975

Purpose

PiggyBac plasmid for synthetic DNA Tape (HEK3 locus)

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC_HA_2xPP7-VP64

Plasmid

#68418

Purpose

Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

PP7(deltaFG)

Use

Synthetic Biology

Tags

HA and VP64

Expression

Mammalian

Mutation

PP7: [delta]67_74 (non-aggregating)

Promoter

human UbC

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pU6_(Gluc)_INT(GenPurpClon)

Plasmid

#68433

Purpose

General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct

Use

CRISPR; Cloning vector for generating int-like co…

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pAc5 HA3-eDHFR-T2A-puro

Plasmid

#86395

Purpose

for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

Depositor

David Bentley

Article

Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

Insert

HA3 eDHFR T2A puro

Use

CRISPR

Tags

HA3 eDHFR T2A puro

Mutation

R12Y, G67S and Y100I mutations in E coli DHFR

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pFLEX+mCyclin D1

Plasmid

#86793

Purpose

mammalian expression of cyclin D1

Depositor

Martine Roussel, Charles Sherr

Article

Matsushime et al Cell. 1991 May 17;65(4):701-13.

Insert

cyclin D1 (Ccnd1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pBluescript-mouse cyclin D2

Plasmid

#86791

Purpose

bacterial expression of mouse cyclin D2

Depositor

Martine Roussel, Charles Sherr

Article

Matsushime et al Cell. 1991 May 17;65(4):701-13.

Insert

cyclin D2 (Ccnd2 Mouse)

Expression

Bacterial

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTorPE-jREX-GECO1

Plasmid

#113941

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jREX-GECO1

Tags

6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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CBP-HAT-GST

Plasmid

#21093

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Tags

GST

Expression

Bacterial

Mutation

HAT domain: aa 1196-1718

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only

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XE107 XPKC alpha-GFP-CS2+

Plasmid

#16743

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

PKC-alpha (prkca Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-CaMKIIbeta(K43R)

Plasmid

#21225

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CaMKIIbeta(K43R) (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

CaMKIIbeta(K43R) Lab plasmid CF 0003.

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWF

Plasmid

#112899

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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