We narrowed to 18,083 results for: She

1. SHC003BSD-DelGFP

   Plasmid

   #133301

   Purpose

   EGFP reporter was removed and the selection marker was modified as blasticidin in the construct of SHC003. This construct could be used for expression of a gene by lentiviral approach.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-CaMKIIbeta(A303R)

   Plasmid

   #21224

   Depositor

   Tobias Meyer

   Article

   Shen et al Science. 1999 Apr 2. 284(5411):162-6.

   Insert

   CaMKIIbeta(A303R) (Camk2b Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CaMKIIbeta(A303R) (Lab plasmid CF 0002)

   Available Since

   July 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5 HA3-eDHFR-T2A-neo

   Plasmid

   #86396

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A neo
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A neo

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. HBT-GCaMP6-HA

   Plasmid

   #107433

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   GCaMP6S
   

   Tags

   HA

   Expression

   Bacterial

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30a-FluB-NP

   Plasmid

   #165093

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Influenza B virus(B/Georgia/05/2019) nucleocapsid protein
   

   Expression

   Bacterial

   Available Since

   Feb. 11, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. RVD-AA5

   Plasmid

   #173240

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Insert

   NI-NI dimer RVD at Position5
   

   Use

   Unspecified

   Mutation

   no

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. RVD-AA9

   Plasmid

   #173244

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Insert

   NI-NI dimer RVD at Position9
   

   Use

   Unspecified

   Mutation

   no

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-MTS-ccdb-DddA(G1333C)-UGI-Puro

   Plasmid

   #173228

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-MTS-ccdb-DddA(G1333N)-UGI-Puro

   Plasmid

   #173229

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. dFP-mini

    Plasmid

    #120543

    Purpose

    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

    Depositor

    Brian Chow

    Article

    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

    Insert

    de novo fluorescence-activating protein mini (dFP-mini)
    

    Tags

    6xHis - TEV cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. punc-119cR

    Plasmid

    #21596

    Depositor

    Al Fisher

    Article

    Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

    Insert

    unc-119:mCherry (unc-119 Nematode)
    

    Use

    Adds unc-119 marker to amp resistant plasmids

    Tags

    mCherry

    Expression

    Worm

    Mutation

    N/A

    Available Since

    Aug. 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-RABIF-FLAG

    Plasmid

    #135558

    Purpose

    Express human RABIF in mammalian cells, FLAG at N terminal

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    RABIF (RABIF Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    T7 promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SHC003BSD

    Plasmid

    #133300

    Purpose

    The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pU1/Sm/3' Box_(GLuc)_TOP1

    Plasmid

    #68437

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U1

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. FusionRed-C-Src-7

    Plasmid

    #56107

    Purpose

    Localization: Membrane/Vesicles, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    C-Src
    

    Tags

    FusionRed

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. CBP-HAT-pET

    Plasmid

    #21091

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    CBP (Crebbp Mouse)
    

    Tags

    His and T7 tag

    Expression

    Bacterial

    Mutation

    HAT domain aa 1171-1621

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pTORKm43GWC

    Plasmid

    #112898

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

    Type

    Empty backbone

    Use

    Oomycete expression

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Phat2-hs-cPla2-C2-EGFP-GH

    Plasmid

    #187088

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSG00000116711 (PLA2G4A Human)
    

    Tags

    EGFP

    Expression

    Bacterial

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV/3' Box_(GLuc)_TOP2

    Plasmid

    #68435

    Purpose

    Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP2 construct
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6_(GLuc)_INT[Spinach2]

    Plasmid

    #68430

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the "Spinach2" aptamer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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