We narrowed to 73,379 results for: LAS

1. pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast

   Plasmid

   #213826

   Purpose

   Expresses human PRDM6 with a C-terminal V5 epitope tag.

   Depositor

   Bjoern Schwer

   Article

   Schmidt et al bioRxiv. 2023 Aug 31:2023.08.29.555389.

   Insert

   PRDM6 (PRDM6 Human)
   

   Use

   Lentiviral and Luciferase

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

   Plasmid

   #63170

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-OLLAS"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

   Plasmid

   #163063

   Purpose

   Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   CheRiff
   

   Use

   Gateway entry vector

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR BFP-guide1

   Plasmid

   #118161

   Purpose

   CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the BFP CDS

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

   Plasmid

   #114091

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a (E174R/S542R)
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   E174R and S542R

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

   Plasmid

   #101236

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K918A/V922A/R925A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K918A, V922A, R925A and Q926A

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

   Plasmid

   #101235

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant F491A/M495A/T496A/N497A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   F491A, M495A, T496A, N497A and Q926A

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

   Plasmid

   #101234

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant T657A/G658A/W659A/R661A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   T657A, G658A, W659A, R661A and Q926A

   Promoter

   T7

   Available since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

   Plasmid

   #101220

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A and Q926A

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid

    #101232

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G528A, V583A, E584A, D585A, N588A and Q926A

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. JDW 1473 (pLenti-BLAST-ZIC3-P2A-H2A-mCherry)

    Plasmid

    #233547

    Purpose

    A CMV driven human ZIC3 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    ZIC3 (ZIC3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A

    Plasmid

    #169826

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1406A S1859A; TCCC -> AGTC silent mutat…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXS-IRES-BLAST flag-CAD delta Loop1 and Loop2

    Plasmid

    #169833

    Purpose

    Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 and F1366 - P1376 in allosteric domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Deletions of amino acids R1398 - S1407 and F1366 …

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXS-IRES-BLAST flag-CAD delta Allosteric Domain

    Plasmid

    #169835

    Purpose

    Expresses C-terminal flag-tagged CAD with substitution of allosteric domain F1308 - C1455 with a (GGGS)X3 linker

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Substitution of amino acids F1308 - C1455 with a …

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-HLP-SACas9-HA-OLLAS-spA

    Plasmid

    #206860

    Purpose

    An AAV plasmid with U6 promoter driving a gRNA against LDLR and liver specific HLP promoter driving SaCas9

    Depositor

    William Lagor

    Article

    De Giorgi et al Methods Mol Biol. 2022;2419:673-713. doi

    Insert

    mLdlr gRNA (Ldlr Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXS-IRES-BLAST flag-CAD R1398A R1403A R1404A

    Plasmid

    #188134

    Purpose

    Expresses C-terminal flag-tagged human CAD R1398A R1403A R1404A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXS-IRES-BLAST flag-CAD E1367A E1368A E1373A

    Plasmid

    #188144

    Purpose

    Expresses C-terminal flag-tagged human CAD E1367A E1368A E1373A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide4

    Plasmid

    #125459

    Purpose

    CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide3

    Plasmid

    #125458

    Purpose

    CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR ZFAND3-guide2

    Plasmid

    #125457

    Purpose

    CRISPR-mediated repression of ZFAND3. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge