We narrowed to 62,057 results for: ica;

1. pENO1-iRFP-NATr

   Plasmid

   #129731

   Purpose

   Integration plasmid to overexpress iRFP in Candida albicans

   Depositor

   Robert Wheeler

   Article

   Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

   Insert

   iRFP-Candida albicans codon-optimized
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ENO1 from Candida albicans

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LOTUS-V/pcDNA3

   Plasmid

   #87127

   Purpose

   Bioluminescent and ratiometric voltage indicator

   Depositor

   Takeharu Nagai

   Article

   Inagaki et al Sci Rep. 2017 Feb 13;7:42398. doi: 10.10

   Insert

   LOTUS-V
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tom20-CIB-stop

   Plasmid

   #117243

   Purpose

   Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

   Depositor

   Robert Hughes

   Article

   Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

   Insert

   CIBN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT3-Neo

   Plasmid

   #69123

   Purpose

   Sleeping Beauty (SB) transposon with G418 resistance

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Insert

   Neo (codon optimized for human)
   

   Use

   Sleeping beauty transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U3MSCV

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET/LEGH

   Plasmid

   #108226

   Purpose

   Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

   Depositor

   Didier Picard

   Article

   Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

   Insert

   LBT-EGFP-His6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7, lacO

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSopBC460S

   Plasmid

   #112514

   Purpose

   PsopB driven sopB (catalytically inactive) and sigE

   Depositor

   Olivia Steele-Mortimer

   Article

   Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

   Insert

   sopB (catalytically inactive)/sigE operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   SopB C460S

   Promoter

   PsopB

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET11a-TAX-L10-STEPtag

   Plasmid

   #159375

   Purpose

   Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   TAX-L10-STEPtag
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET11a-STEPtag-L10-TAX

   Plasmid

   #159374

   Purpose

   Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   STEPtag-L10-TAX
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST96/LBT-MCS

   Plasmid

   #108227

   Purpose

   Bacterial expression expression of protein with N-terminal lanthanide binding tag

   Depositor

   Didier Picard

   Article

   Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

   Insert

   LBT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7, lacO

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pADH118-17

    Plasmid

    #91727

    Purpose

    NAT-marked C. albicans URA3-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2of2, pSNR52, C. albicans URA3-specific gRNA, LEU2 2of2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

    Plasmid

    #217478

    Purpose

    Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

    Depositor

    Peng Zou

    Article

    Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

    Insert

    Nier1s
    

    Use

    Tags

    3xNLS, P2A-EGFP-3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

    Plasmid

    #217479

    Purpose

    Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

    Depositor

    Peng Zou

    Article

    Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

    Insert

    Nier1b
    

    Use

    Tags

    3xNLS, P2A-EGFP-3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET16b-RPA4/His14 (MSW#949)

    Plasmid

    #208075

    Purpose

    Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

    Depositor

    Marc Wold

    Article

    Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

    Insert

    RPA4, RPA14(His-tagged)
    

    Use

    Tags

    10xHis tag on RPA14

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET3a-FruK

    Plasmid

    #186256

    Purpose

    Overexpression of FruK for purification

    Depositor

    Emile Van Schaftingen, Liskin Swint-Kruse

    Article

    Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

    Insert

    1-phosphofructokinase (fruK E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-NES-KIRIN1

    Plasmid

    #107416

    Purpose

    Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1
    

    Use

    Tags

    Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB_mU6_enh-gRNA_Puro-T2A-BFP

    Plasmid

    #235571

    Purpose

    Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    sgRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. FokI-dCas9

    Plasmid

    #52970

    Purpose

    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

    Depositor

    David Liu

    Article

    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Fok1 fused to dCas9
    

    Use

    CRISPR

    Tags

    Fok1 nuclease (homodimeric wildtype) and NLS

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    CMV

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3-Neo-EF1a-GFP

    Plasmid

    #69134

    Purpose

    Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

    Depositor

    Martin Bonamino

    Article

    Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

    Type

    Empty backbone

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U3MSCV

    Available since

    Jan. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTA-HUH

    Plasmid

    #221152

    Purpose

    HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

    Depositor

    Sheng Yang

    Article

    Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

    Insert

    HisG-URA3-HisG
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS156

    Plasmid

    #182707

    Purpose

    CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

    Depositor

    Rebecca Shapiro

    Article

    Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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