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pFETCh_SSRP1
Plasmid#72370PurposeDonor vector for 3' FLAG tag of human SSRP1DepositorInsertSSRP1 Homology Arms (SSRP1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
GuideVector_BbsI
Plasmid#110692PurposeFor rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-SpCas9-P2A-blast
Plasmid#106477Purposesingle-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKSB-618 U6-sgRNA2/3 GGAA AACA
Plasmid#173674PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB-631 U6-sgRNA3/4 AACA GCTT
Plasmid#173675PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6-BbsI-crRNA
Plasmid#51026PurposeGenerates crRNA for use in combination with tracrRNADepositorInsertU6-BbsI-crRNA
UseCRISPRTagsExpressionInsectMutationPromoterU6-2Available sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF219
Plasmid#72367PurposeDonor vector for 3' FLAG tag of human ZNF219DepositorInsertZNF219 Homology Arms (ZNF219 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_TGIF2
Plasmid#72373PurposeDonor vector for 3' FLAG tag of human TGIF2DepositorInsertTGIF2 Homology Arms (TGIF2 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF146
Plasmid#72359PurposeDonor vector for 3' FLAG tag of human ZNF146DepositorInsertZNF146 Homology Arms (ZNF146 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-HeFSpCas9-H840A
Plasmid#80456Purposehigh fidelity enhanced SpCas9 mammalian expression vectorDepositorInsertHeFSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailable sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
1203AB-CMV-Cas7.1-H43A-mutant
Plasmid#221541PurposeEffector expresses Cas7.1 (truncated from DiCas7-11), H to A mutation at amino acid 43DepositorInsertTruncated DiCas7-11
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203AC-CMV-Cas7.1-Y55A-mutant
Plasmid#221542PurposeEffector expresses Cas7.1 domain (truncated from DiCas7-11), Y to A mutation at amino acid 55DepositorInsertTruncated DiCas7-11
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203AD-CMV-Cas7.1-N152A-mutant
Plasmid#221543Purposeffector expresses Cas7.1 domain (truncated from DiCas7-11), N to A mutation at amino acid 152DepositorInsertTruncated DiCas7-11
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTD56
Plasmid#172895PurposemKate2^SEC^AID* vector with ccdB markers for cloning homology armsDepositorInsertmKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTD72
Plasmid#172896PurposemKate2^SEC^2xHA vector with ccdB markers for cloning homology armsDepositorInsertmKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_PBX2
Plasmid#72356PurposeDonor vector for 3' FLAG tag of human PBX2DepositorInsertPBX2 (PBX2 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458-AAVS1
Plasmid#113194PurposeEncodes gRNA for human AAVS1DepositorUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
EC64420
Plasmid#209517PurposeCloning in complete guide expression cassettes, with Cas9DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC70364
Plasmid#209515PurposeCloning in complete guide expression cassettes, with Cas9DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only
