We narrowed to 24,391 results for: nes

1. pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)

   Plasmid

   #164193

   Purpose

   Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)

   Depositor

   Thomas Oertner

   Article

   Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

   Inserts

   CamPARI2
   Human α Tubulin
   

   Use

   AAV

   Tags

   6 His tag and NES

   Expression

   Mammalian

   Mutation

   F391W-L398V

   Promoter

   Syn

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pvha6-AgDD

   Plasmid

   #80626

   Purpose

   create protein aggregates in C. elegans

   Depositor

   Thomas Wandless

   Article

   Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

   Insert

   NES-L106P-sfGFP (FKBP1A Human)
   

   Use

   Tags

   MLALKLAGLDI

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1_jRCaMP1b

   Plasmid

   #183758

   Purpose

   Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   jRCaMP1b
   

   Use

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-AMPKsub-YPet-Kras

   Plasmid

   #84638

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub-YPet-Kras
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ARB344

   Plasmid

   #124027

   Purpose

   Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic iRFP713

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   L1-B1_CMV-NES::iRFP713-rglPA_R2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. ARB345

   Plasmid

   #124028

   Purpose

   Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic tagBFP

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   L1-B1_CMV-NES::tagBFP-rglPA_R2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. ARB346

   Plasmid

   #124029

   Purpose

   Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mAzamiGreen

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   L1-B1_CMV-NES::mAzamiGreen-rglPA_R2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ARB347

   Plasmid

   #124030

   Purpose

   Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mRuby2

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   L1-B1_CMV-NES::mRuby2-rglPA_R2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. JPF0455

   Plasmid

   #124040

   Purpose

   Encodes pPGK1 driving expression of cytoplasmic tagBFP in a 2nd gen. Lenti Virus destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pHR_pPGK1-NES-tagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWMD23

    Plasmid

    #140006

    Purpose

    Expression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Inserts

    ORF1 Shuffle 1 NLS
    Pong TPase LA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    NES sequence mutated L418A, L420A and Ping N-term…

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bxb1_AJMA

    Plasmid

    #183760

    Purpose

    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Inserts

    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    

    Use

    Tags

    Lck and NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSK267

    Plasmid

    #129272

    Purpose

    "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    NLS-VP16-ZF 43-8-NES
    

    Use

    Tags

    6xHA

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3/ TO myc-cCE-bio (cCE-bio)

    Plasmid

    #82473

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form

    Depositor

    Daniel Schoenberg

    Article

    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

    Insert

    Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Synthetic, Mouse)
    

    Use

    Tags

    TEV-Biotin and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSAM_AraC

    Plasmid

    #91569

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Insert

    AraC/PBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMR150

    Plasmid

    #32292

    Depositor

    Verne Chapman, Rudolf Jaenisch

    Article

    Sanford et al Genes Dev. 1987 Dec;1(10):1039-46.

    Insert

    genomic methylation probe-minor satellite repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAOJ15

    Plasmid

    #91567

    Purpose

    Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tetracycline promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGM6

    Plasmid

    #110660

    Purpose

    Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.

    Depositor

    David Russell

    Article

    Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.

    Insert

    AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWC2

    Plasmid

    #15960

    Depositor

    Jeff Gelles

    Article

    Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

    Insert

    Kinesin 1-401 BCCP his tag (Khc E. coli, Fly)
    

    Use

    Tags

    BCCP and His6

    Expression

    Bacterial

    Mutation

    amino acids 1-401

    Promoter

    Available since

    Nov. 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTTR210K

    Plasmid

    #41750

    Depositor

    Jeff Gelles

    Article

    Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

    Insert

    kinesin heavy chain (Khc E. coli, Fly)
    

    Use

    Tags

    His6 and bccp

    Expression

    Bacterial

    Mutation

    residues 1-401; R210K

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pTTSTOP

    Plasmid

    #41751

    Depositor

    Jeff Gelles

    Article

    Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

    Insert

    kinesin heavy chain (Khc Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues 1-401

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only