We narrowed to 2,475 results for: T2A

1. pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert

   Plasmid

   #111153

   Purpose

   knock in bait for Axolotl Pax7 locus

   Depositor

   Elly Tanaka

   Article

   Fei et al Proc Natl Acad Sci U S A. 2017 Nov 21;11

   Insert

   GAP43-Cherry-T2a-mERt-Cre-Ert
   

   Use

   Cre/Lox

   Mutation

   wt

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. 874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159672

   Purpose

   Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   βTub-NLS-hSpCas9-T2A-GFP
   

   Tags

   T2A-GFP

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

   Plasmid

   #170183

   Purpose

   Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   sgBFP
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159671

   Purpose

   Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   ExuL-NLS-hSpCas9-T2A-GFP
   

   Tags

   T2A-GFP

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

   Plasmid

   #89994

   Purpose

   Donor template for generation of NGN3-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159673

   Purpose

   Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
   

   Tags

   T2A-GFP

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

   Plasmid

   #121175

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

   Plasmid

   #172916

   Purpose

   Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7s-T2A-tdTomato
   

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron

   Plasmid

   #246435

   Purpose

   Expresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS.

   Depositor

   Ailong Ke

   Article

   Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

   Inserts

   O.gue IscB with dead mutations (D60A, H269A) and delta-TID
   EGFP
   

   Tags

   SV40 NLS, Thosea asigna virus 2A peptide, and nuc…

   Expression

   Mammalian

   Mutation

   changed Aspartic Acid 60 to Alanine, changed Hist…

   Promoter

   CMV

   Available Since

   Oct. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. XZ066-(PB)EF1a-LIDARST-CCR6V2-IRESv0-T2A-PuroR-P2A-BFP

    Plasmid

    #233418

    Purpose

    PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
    

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available Since

    June 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)

    Plasmid

    #238167

    Purpose

    Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    Non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-[EF1a-mRuby2-bGH]-UbC-PuroR-T2A-mGL-SV40pA

    Plasmid

    #235325

    Purpose

    PiggyBac integration vector for ComMAND base gene regulating mRuby2

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    EF1a (human EF1a)

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

    Plasmid

    #234737

    Purpose

    All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

    Depositor

    Kirill Martemyanov

    Article

    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

    Insert

    Nrl (Nrl Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP

    Plasmid

    #196990

    Purpose

    Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

    Insert

    VEGFR2
    

    Use

    CRISPR and Lentiviral

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

    Plasmid

    #231910

    Purpose

    For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

    Plasmid

    #218916

    Purpose

    Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
    

    Use

    Lentiviral

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT256 Lenti pEF-dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #187332

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT257 Lenti pEF-dCas9-3XFLAG-Z+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #187333

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-Z+F+N-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJT255 Lenti pEF-dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187331

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJT331 Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #195538

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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