We narrowed to 22,588 results for: his

Showing: 1301 - 1320 of 22588 results

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CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pET MBP mCherry LIC cloning vector (MBP-mCherry)

Plasmid

#29747

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

Expression

Bacterial

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pST-KT

Plasmid

#44561

Purpose

for ectopic inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Promoter

UV15 promoter

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4-FLAG-Jhdm2a

Plasmid

#38136

Depositor

Toshinobu Nakamura, Toru Nakano

Article

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Insert

Jhdm2a (Kdm3a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pUCBB-eGFP

Plasmid

#32548

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pET15b-KSRP

Plasmid

#23019

Depositor

Douglas Black

Article

Min et al Genes Dev. 1997 Apr 15. 11(8):1023-36.

Insert

KSRP (KHSRP Human)

Tags

His

Expression

Bacterial

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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pBad-mAmetrine1.1

Plasmid

#18084

Purpose

Bacterial expression of mAmetrine (version 1.1) fluorescent protein

Depositor

Robert Campbell

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Insert

mAmetrine1.1

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pAcGP67A-PSMA

Plasmid

#11524

Depositor

Pamela Bjorkman

Article

Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 1

Insert

prostate-specific membrane antigen (FOLH1 Human)

Tags

His

Expression

Insect

Mutation

There is a short N-terminal truncation of this pr…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pET32a-TRXtag

Plasmid

#11516

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

None - thioredoxin is vector encoded (trxA )

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

vector modified; removed N-terminal His tag by di…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

Plasmid

#101063

Purpose

AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2_F391W-G395D

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mSTIM1 myc

Plasmid

#17732

Depositor

Anjana Rao

Article

Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Insert

STIM1 (Stim1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2S

Plasmid

#15807

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2S (UBE2S Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4-Myc-HisB-Sirt3

Plasmid

#24918

Depositor

Toren Finkel

Article

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPP085

Plasmid

#158795

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SnoopTag-mEGFP-SpyTag

Plasmid

#72325

Purpose

Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SnoopTag-mEGFP-SpyTag

Tags

N-terminal His6 tag

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pQE-80L MBP-SspB Nano

Plasmid

#60409

Purpose

E. coli expression of SspB Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

SspB nano

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

Y11K and A15E

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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hUbc13

Plasmid

#51131

Purpose

human ubc13, E2 for synthesis k63 linked ubiquitin chain

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

ubc13 (UBE2N Human)

Tags

His6 and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pfmh-hCry1

Plasmid

#25843

Depositor

Aziz Sancar

Insert

human Cryptochrome 1 (CRY1 Human)

Tags

flag, his, and myc

Expression

Mammalian

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only