We narrowed to 5,839 results for: pCas

1. pCaSpeR-act5cB-LexAQF

   Plasmid

   #61310

   Purpose

   Expresses chimeric transactivator LexA_DBD:QF2_AD in Drosophila

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   LexA_DBD::QF2_AD
   

   Expression

   Insect

   Available Since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS/EG5-RBD

   Plasmid

   #213410

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/EG5-RBD (S )
   

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_ACTB-i1 sgRNA / hSpCas9

   Plasmid

   #172825

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-SP-CLSTN2-3xHAtag

   Plasmid

   #191693

   Purpose

   For expression coding sequencing of CLSTN2

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   signal peptide, full length coding sequence of CLSTN2 and 3xHAtag
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-AILR-LplA-ER

   Plasmid

   #42574

   Depositor

   Alice Ting

   Article

   Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

   Insert

   LplA(AILR) with ER-retention sequence KDEL
   

   Tags

   FLAG

   Mutation

   Compared to wild-type LplA: tryptophan 37 to alan…

   Promoter

   CAG

   Available Since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

   Plasmid

   #107727

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-roxSTOProx-LynEGFP

   Plasmid

   #133923

   Purpose

   Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Dre-based recombination

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   LynEGFP
   

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cav2.2 D122A HA pCAGGS

   Plasmid

   #206097

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

   Depositor

   Annette Dolphin

   Article

   Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Expression

   Mammalian

   Mutation

   D122A

   Promoter

   CMV/B-actin

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG Fzd7 GFP

   Plasmid

   #66106

   Purpose

   expression of chicken Fzd7 GFP

   Depositor

   Felicitas Proels, Martin Scaal

   Article

   et al Development. 2015 Feb 15;142(4):665-71.

   Insert

   Fzd7 (FZD7 Chicken)
   

   Use

   Chicken expression

   Tags

   EGFP

   Promoter

   CAG

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-MCS-DsRed

    Plasmid

    #164091

    Purpose

    expression of DsRed-tagged proteins under CAG promoter

    Depositor

    Maik Hintze

    Insert

    DsRed
    

    Tags

    DsRed

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAFNF-PSDd1.2-GFP

    Plasmid

    #125581

    Purpose

    Flp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    PSDd1.2-GFP (Dlg4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-EGFP-Snapin

    Plasmid

    #118743

    Purpose

    Expresses EGFP tagged Snapin

    Depositor

    Beverly Koller

    Insert

    Snapin cDNA (Snapin Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458-3xHA-SpCas9

    Plasmid

    #130968

    Purpose

    3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    3xHA-NLS-SpCas9
    

    Use

    CRISPR

    Tags

    3x HA, EGFP, and NLS

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT316_pattB-pCA-GFP-pA

    Plasmid

    #52548

    Purpose

    for Phic31 integrase-mediated transgene integration

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCaSpeR-act5cB-QF#7m1

    Plasmid

    #46126

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7m1
    

    Expression

    Insect

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-LSS-mOrange

    Plasmid

    #89688

    Purpose

    pCAG promoter driving LSS-mOrange

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    LSS-mOrange
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-ArCas12a-2AeGFP

    Plasmid

    #123632

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    ArCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9(1.1)_No_FLAG_ATP1A1-G7

    Plasmid

    #173203

    Purpose

    Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCASP-SptP120-TD-HilA

    Plasmid

    #153330

    Purpose

    Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-HA4-7c12 Tandem Monobody
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459V2.0-SpCas9-HF4

    Plasmid

    #108295

    Purpose

    pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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