We narrowed to 5,839 results for: pCas

1. pCAG-AviMta3-3xFiBsd

   Plasmid

   #140962

   Purpose

   full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

   Depositor

   Brian Hendrich

   Article

   Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

   Insert

   metastasis associated 3 (Mta3 Mouse, Synthetic)
   

   Tags

   3xFLAG, Avi, and TEV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-Flag-dHeFSpCas9

   Plasmid

   #92116

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   Cbh

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG eGFP-Oct6

   Plasmid

   #203790

   Purpose

   Competitive assay of Oct4 and Oct6 binding to DNA motifs.

   Depositor

   Thorsten Wohland

   Article

   Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

   Insert

   Pou3f1 (Pou3f1 Mouse)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CpcB-Solanum-lycopersicumPHLS_CpcA

   Plasmid

   #74003

   Purpose

   Expresses the Solanum lycopersicum PHLS as a fusion with the CpcB protein plus the NPPS and CmR cassete in Synechocystis

   Depositor

   Anastasios Melis

   Article

   Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

   Inserts

   PHLS and CmR
   Neryl diphosphate synthase from Solanum lycopersicum
   

   Promoter

   cpc

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-S-B.1.1.7-FLAG

   Plasmid

   #177093

   Purpose

   B.1.1.7 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein B.1.1.7 (S SARS-CoV-2)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

   Plasmid

   #107730

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

   Plasmid

   #107729

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW829_pCAG-pExpr-LUT

   Plasmid

   #87554

   Purpose

   Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. BII-gR-PtW-eSpCas9

   Plasmid

   #133357

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Tags

   HA-tagged eSpCas9

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-2XHA-Borcs7

    Plasmid

    #118747

    Purpose

    Expresses 2XHA tagged Borcs7

    Depositor

    Beverly Koller

    Insert

    Borcs7 cDNA (2010012O05Rik Mouse)
    

    Tags

    2XHA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Scarlet-Borcs7

    Plasmid

    #118750

    Purpose

    Expresses scarlet tagged Borcs7-Q87X

    Depositor

    Beverly Koller

    Insert

    Borcs7-Q87X cDNA (2010012O05Rik Mouse)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Q87X

    Promoter

    CAG

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-TetR-Rad18UBD

    Plasmid

    #119010

    Purpose

    Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    TetR
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-BsCas12a-2AeGFP

    Plasmid

    #123633

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    BsCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCaSpeR eGFP-dhd

    Plasmid

    #113004

    Purpose

    P element vector for transposon insertion into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Insert

    dhd (dhd Fly)
    

    Use

    P insertion vector

    Tags

    eGFP

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCaSpeR dhd-eGFP

    Plasmid

    #113003

    Purpose

    P element vector for transposon insertion of dhd-eGFP into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Insert

    dhd (dhd Fly)
    

    Use

    P insertion vector

    Tags

    eGFP

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Trex2-bpA

    Plasmid

    #86984

    Purpose

    Expression of mouse Trex2

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Insert

    mouse Trex2 coding region (Trex2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9–mKate2ps–EgRNA

    Plasmid

    #62716

    Purpose

    Empty; no U6 gRNA cassette

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Expression

    Mammalian

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-PrCas12a-2AeGFP

    Plasmid

    #123636

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    PrCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCAS9n(BB)-2A-puro DNM1, A

    Plasmid

    #107795

    Purpose

    gRNA vector for knock-in gene editing at human DNM1 locus

    Depositor

    Sandra Schmid

    Article

    Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

    Insert

    gRNA targeting human DNM1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCAS9n(BB)-2A-puro DNM1, B

    Plasmid

    #107796

    Purpose

    gRNA vector for knock-in gene editing at human DNM1 locus

    Depositor

    Sandra Schmid

    Article

    Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

    Insert

    gRNA targeting human DNM1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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