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Showing: 2681 - 2720 of 21995 results

  3. pXGFPC-5 Pxyl-dcas9 (Spy)

    Plasmid
    #133316
    Purpose
    expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus pyogenes)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    xylose
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    133316-plasmid-map-sequence-id-266825

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  7. pGBW-m4046925

    Plasmid
    #145694
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145694-plasmid-map-sequence-id-313545

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  13. pBICI_2_strong

    Plasmid
    #155168
    Purpose
    see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155168-plasmid-map-sequence-id-310553

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  19. Nano-lantern-H2B/pcDNA3

    Plasmid
    #51971
    Purpose
    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B
    Depositor
    Takeharu Nagai
    Article
    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc
    Insert
    Nano-lantern
    Use
    Tags
    human H2B
    Expression
    Mammalian
    Mutation
    S257G in Rluc8
    Promoter
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. p.UTA.2.0-GL-2

    Plasmid
    #82440
    Purpose
    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2
    Depositor
    Jens Gruber
    Article
    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10
    Insert
    Luciferase
    Use
    Tags
    Expression
    Mutation
    The complementary region for GL2 siRNA control wa…
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82440-plasmid-map-sequence-id-168133

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  23. pGBW-m4046952

    Plasmid
    #145718
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145718-plasmid-map-sequence-id-307567

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  25. ERroGFP_iE_C204Q_pCDNA3

    Plasmid
    #48629
    Purpose
    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERroGFP
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    insertion E147B, C204Q
    Promoter
    Available since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48629-plasmid-map-sequence-id-70377

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  26. pGBW-m4046938

    Plasmid
    #145599
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145599-plasmid-map-sequence-id-300265

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  27. ERroGFP_C147S_pCDNA3

    Plasmid
    #48630
    Purpose
    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERroGFP
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    C147S
    Promoter
    Available since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48630-plasmid-map-sequence-id-70378

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  30. ERrefGFP1_pCDNA3

    Plasmid
    #48635
    Purpose
    Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERrefGFP1
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    C147S,C204Q
    Promoter
    Available since
    Oct. 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    48635-plasmid-map-sequence-id-70401

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  36. pcDNA3-mCherry-PKAsub-RLuc8N-NES

    Plasmid
    #138211
    Purpose
    Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    mCherry-PKAsub-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    138211-plasmid-map-sequence-id-307413

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  37. Antares-SEPLuc

    Plasmid
    #141086
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 66 to 91 on the Luciferas…
    Promoter
    CMV
    Available since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    141086-plasmid-map-sequence-id-296862

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  38. pGBW-m4046970

    Plasmid
    #145753
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145753-plasmid-map-sequence-id-311539

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  39. roGFP_iE_C204S_pQE30

    Plasmid
    #48632
    Purpose
    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Serine mutation (roGFP C147S, E147b, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    roGFP1
    Use
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    insertion E147B, c204S
    Promoter
    Available since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48632-plasmid-map-sequence-id-70381

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Showing: 2681 - 2720 of 21995 results