168,238 results

1. pCA24N-p50-ligase

   Plasmid

   #87742

   Purpose

   IPTG-inducible expression of p50-ligase fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Tags

   6xHis and human p50/NF-kB (amino acids 40-366)

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330-mcherry

   Plasmid

   #98750

   Purpose

   Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

   Depositor

   Jinsong Li

   Article

   Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZVsE_SC12

   Plasmid

   #219722

   Purpose

   Zika virus stable E dimer with fusion loop mutation and increased expression

   Depositor

   Brian Kuhlman

   Article

   Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

   Insert

   ZKV sE SC12
   

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A264W, T267R, S286W, S287P, G29K, A25M

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSPL3-hRPH3A_c.444Gwt

   Plasmid

   #190476

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. RNb-GFP

   Plasmid

   #128777

   Purpose

   Mammalian expression

   Depositor

   Colin Taylor

   Article

   Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

   Insert

   RNb-GFP
   

   Expression

   Mammalian

   Available Since

   Feb. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (AAV5)

   Viral Prep

   #100054-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (#100054). In addition to the viral particles, you will also receive purified pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 plasmid DNA. CAG-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CAG

   Tags

   mCherry

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pMH-Halo tag

   Plasmid

   #154144

   Purpose

   Gateway compatible mammalian expression plasmid for halo tag fusion proteins

   Depositor

   Michael Huen

   Type

   Empty backbone

   Tags

   Halo tag

   Expression

   Mammalian

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dual sgRNA CRISPRa Library

   Pooled Library

   #1000000193

   Depositor

   Jonathan Weissman

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

9. pLKO5.sgRNA.EFS.tRFP

   Plasmid

   #57823

   Purpose

   3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP
   

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mini-human AsCpf1-based human genome-wide knockout library

    Pooled Library

    #130630

    Purpose

    Expresses crRNAs for genome-wide knockout pooled library screening

    Depositor

    Giulio Draetta

    Article

    Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only