164,169 results

1. pET-28b-RfxCas13d-His

   Plasmid

   #141322

   Purpose

   Plasmid for bacterial expression and purification of RfxCas13d protein

   Depositor

   Ariel Bazzini, Miguel Angel Moreno-Mateos

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDSP33

   Plasmid

   #199375

   Purpose

   Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   no mutations, except added subcellular localizati…

   Promoter

   Available since

   April 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. AAVS1-Puro CAG FUCCI

   Plasmid

   #136934

   Purpose

   donor plasmid for constitutive FUCCI expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

   Insert

   Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
   

   Use

   Synthetic Biology

   Tags

   Clover and mKO2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-SMVP-Cas9C

   Plasmid

   #80931

   Purpose

   Expresses Cas9C in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EGFPC2-CB2 SH3+

   Plasmid

   #68821

   Purpose

   EGFP tagged Collybistin expression in mammalian cells

   Depositor

   Shiva Tyagarajan

   Article

   de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

   Insert

   Collybistin (Arhgef9 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCherry-Rab5C-S35N

   Plasmid

   #110503

   Purpose

   Dominant Negative

   Depositor

   Bernardo Mainou

   Insert

   Rab5C-S35N (RAB5C Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   S35N

   Promoter

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAC1802_pmax-RBFOX1N-dCasRx-C

   Plasmid

   #118635

   Purpose

   Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   RBFOX1N-dCasRx-C
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL2-Full

   Plasmid

   #16012

   Depositor

   Wafik El-Deiry

   Article

   Takimoto et al Oncogene. 2000 Mar 30. 19(14):1735-43.

   Insert

   KILLER/DR5 (TNFRSF10B Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pHS15 (hANGPTL3-Strep)

   Plasmid

   #109112

   Purpose

   Expresses StrepII-tagged human ANGPTL3 in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

   Insert

   ANGPTL3 (ANGPTL3 Human)
   

   Use

   Tags

   StrepII

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197064

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge