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pET28a-Vx3(A7)

Plasmid

#35526

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3(A7)

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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DS-TDcasN-

Plasmid

#48660

Purpose

Bacterial nuclease-null TD Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
TD tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC and tracrRNA promoter

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-Map7emtb

Plasmid

#46078

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7emtb

Tags

GFP

Expression

Mammalian

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-LexAQF

Plasmid

#61310

Purpose

Expresses chimeric transactivator LexA_DBD:QF2_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA_DBD::QF2_AD

Expression

Insect

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEB1-2ndVal mGFPmut2

Plasmid

#104033

Purpose

Plasmid encoding 2ndVal mGFPmut2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mGFPmut2

Use

Low copy

Expression

Bacterial

Mutation

mGFPmut2 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pEB1-comTurquoise2

Plasmid

#104018

Purpose

Plasmid encoding comTurquoise2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

comTurquoise2

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as mTurquoise2 (codon optim…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAL-pgi_T

Plasmid

#74066

Purpose

pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pgi (T)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
pJL1121

Plasmid

#78188

Purpose

intestinal GFP expression by fasting in worms

Depositor

Junho Lee

Article

Jo et al Cell Metab. 2009 May;9(5):440-8. doi: 10

Insert

fil-2 (fil-2 Nematode)

Expression

Worm

Promoter

fil-2

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pEB1-comCerulean3

Plasmid

#104017

Purpose

Plasmid encoding comCerulean3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

comCerulean3

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as mCerulean3 (codon optimi…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAND_Reporter

Plasmid

#60591

Purpose

AND gate reporter

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_Reporter

Expression

Bacterial

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pEB1-coCeruleanME

Plasmid

#104016

Purpose

Plasmid encoding coCeruleanME

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coCeruleanME

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as Cerulean ME (codon optim…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDS282

Plasmid

#34983

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

cpPDZ–mCherry

Expression

Yeast

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAP997-1

Plasmid

#99500

Purpose

3XFlag::tagRFP::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagRFP::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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hgRNA-A21_pLKO-Puro

Plasmid

#100570

Purpose

hgRNA-A21 in a lentiviral backbone with puromycin resistance

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-A21

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal Kat9-5

Plasmid

#104031

Purpose

Plasmid encoding 2ndVal Katushka9-5

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal Katushka9-5

Use

Low copy

Expression

Bacterial

Mutation

Katushka9-5 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mCherry-L

Plasmid

#104026

Purpose

Plasmid encoding 2ndVal mCherry-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mCherry-L

Use

Low copy

Expression

Bacterial

Mutation

mCherry-L with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-coSCFP1

Plasmid

#104014

Purpose

Plasmid encoding coSCFP1

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coSCFP1

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as SCFP1 (codon optimized n…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-comVenJBC

Plasmid

#104020

Purpose

Plasmid encoding comVenJBC

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

comVenJBC

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as mVenus JBC (codon optimi…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-comVenME

Plasmid

#104021

Purpose

Plasmid encoding comVenME

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

comVenME

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as mVenus ME (codon optimiz…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-coClover

Plasmid

#104022

Purpose

Plasmid encoding coClover

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coClover

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as Clover (codon optimized …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-comClover3

Plasmid

#104023

Purpose

Plasmid encoding comClover3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

comClover3

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as mClover3 (codon optimize…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAP975-1

Plasmid

#99498

Purpose

TEV::linker::tagBFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagBFP::3XFlag

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAND_Int2

Plasmid

#60590

Purpose

A transcriptional AND circuit constructed by layering NOR and NOT gates

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_Int2

Expression

Bacterial

Promoter

PPhlF

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAP758-1

Plasmid

#99494

Purpose

TEV::eGFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::3XFlag

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP546-2

Plasmid

#99476

Purpose

3XFlag::mNeptune::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::mNeptune::Myc

Expression

Bacterial

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAND_yfp

Plasmid

#60592

Purpose

used with pSpec to generate an AND gate without memory

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_yfp

Expression

Bacterial

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PM-TD!TB

Plasmid

#48656

Purpose

Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGH314_wtGFP S65T, Q80H

Plasmid

#85407

Purpose

lentiviral expression vector for wtGFP S65T-Q80H

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP S65T, Q80H

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBPHLWL-QF

Plasmid

#26269

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

QF (qf Neurospora crassa)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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FISSEQ-hgRNA-Design2

Plasmid

#100572

Purpose

FISSEQ-hgRNA-Design2 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

FISSEQ-hgRNA-Design2

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpec

Plasmid

#60593

Purpose

used with pAND_YFP to generate an AND gate without memory

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Spec

Expression

Bacterial

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-2ndVal mRFP1

Plasmid

#104025

Purpose

Plasmid encoding 2ndVal mRFP1

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mRFP1

Use

Low copy

Expression

Bacterial

Mutation

mRFP1 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-15-LexABD-G4MD-QFAD

Plasmid

#46105

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexABD-G4MD-QFAD

Expression

Insect

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
SK-YFP-TD-B

Plasmid

#48663

Purpose

Bacterial TD repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer B/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCis_7+8+10

Plasmid

#60588

Purpose

a 3-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_7+8+10

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCis_7+8

Plasmid

#60587

Purpose

a 2-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_7+8

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
EMX1_sgRNA

Plasmid

#100555

Purpose

Expresses EMX1 sgRNA. Target sequence: GAGTCCGAGCAGAAGAAGAA

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

EMX1 sgRNA

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PM-NM!TA

Plasmid

#48651

Purpose

Bacterial NM crRNA expression: targets NM to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Phamret/pcDNA3

Plasmid

#51953

Purpose

mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein.

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

Phamret

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

Showing: 2721 - 2760 of 26841 results

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