Skip to main content
Addgene
Showing: 3401 - 3450 of 49469 results

  3. pcDNA3.1_Tau Authentic Alu 10-12K317M

    Plasmid
    #194168
    Purpose
    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau K317M mutant. Expresses tau circular RNA 12-->10 KM with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 K317M
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Lysine 317 to Methionine
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194168-plasmid-map-sequence-id-381799

    Enlarge

  5. pEGFP-N1-GRASP55

    Plasmid
    #137708
    Purpose
    Eukaryotic expression of rat GRASP55 full-length
    Depositor
    Yanzhuang Wang
    Insert
    GRASP55
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    137708-plasmid-map-sequence-id-273547

    Enlarge

  8. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3Ă—FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

    Enlarge

  9. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3Ă—FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71286-plasmid-map-sequence-id-127777

    Enlarge

  14. S10A mutant of H3S10ph biosensor

    Plasmid
    #120810
    Purpose
    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability
    Depositor
    Yingxiao Wang
    Article
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Insert
    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Serine 10 is mutated to Alanine, and Threonine 3,…
    Promoter
    CMV
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120810-plasmid-map-sequence-id-235276

    Enlarge

  15. FLINC-AKAR1(TA)-CAAX

    Plasmid
    #87706
    Purpose
    Negative-control mutant of FLINC-AKAR1-CAAX.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    FLINC-AKAR1(TA)-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87706-plasmid-map-sequence-id-171088

    Enlarge

  16. PKAsub-TagRFPT-CAAX

    Plasmid
    #87712
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub-TagRFPT-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87712-plasmid-map-sequence-id-176157

    Enlarge

  19. PKAsub(TA)-TagRFPT-CAAX

    Plasmid
    #87740
    Purpose
    Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub(TA)-TagRFPT-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87740-plasmid-map-sequence-id-176201

    Enlarge

  26. KBB528

    Plasmid
    #185104
    Purpose
    C-terminal truncation of Pom152 fused to GFP
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    POM152(1-???)-HA-GFP
    Promoter
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185104-plasmid-map-sequence-id-368165

    Enlarge

  27. LDB361

    Plasmid
    #185435
    Purpose
    Bacterial expression of full length NUP2 as fusion with GST
    Depositor
    Laura Davis, Ken Belanger
    Insert
    NUP2
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185435-plasmid-map-sequence-id-367560

    Enlarge

  28. KBB609

    Plasmid
    #185110
    Purpose
    Pom152 N-terminus in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 N-term-6His
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185110-plasmid-map-sequence-id-368108

    Enlarge

  29. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3Ă—FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

    Enlarge

  30. pGL4.22-PGK1-HRE::dLUC

    Plasmid
    #128095
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Mutation
    Promoter
    inserted PGK1 hypoxia response elements (HRE) (x3)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128095-plasmid-map-sequence-id-249667

    Enlarge

  31. pT3-EF1aH-PIK3CA H1047R

    Plasmid
    #180031
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.
    Depositor
    Xin Chen
    Article
    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv
    Insert
    PIK3CA H1047R (PIK3CA Human)
    Use
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    H1047R
    Promoter
    EF1a
    Available since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180031-plasmid-map-sequence-id-357864

    Enlarge

  34. XLone-Puro beta-catenin P2A eGFP-NLS

    Plasmid
    #155189
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP
    Depositor
    Xiaoping Bao
    Article
    Chang et al Biomaterials. 2022 May 6;285:121569. doi
    Insert
    beta-catenin (Ctnnb1 Mouse)
    Use
    Unspecified; Piggybac transposon
    Tags
    P2A eGFP NLS
    Expression
    Mammalian
    Mutation
    Sites phosphorylated by GSK-3beta have been mutat…
    Promoter
    TRE3G
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    155189-plasmid-map-sequence-id-307963

    Enlarge

  38. 7AA-aSyn-pRK172

    Plasmid
    #236197
    Purpose
    This plasmid expresses human α-synuclein with a 7-amino acid insertion (MAAAEKT) in the pRK172 backbone for bacterial expression in E. coli. The insertion corresponds to the JOS mutation.
    Depositor
    Yang Yang, Sjors Scheres
    Article
    Yang et al Acta Neuropathol. 2023 May;145(5):561-57
    Insert
    SNCA (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    insertion os MAAAEKT at residue 22
    Promoter
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236197-plasmid-map-sequence-id-469796

    Enlarge

  40. KBB610

    Plasmid
    #185111
    Purpose
    Pom152 full repeat domain in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 Repeats-6His
    Promoter
    Available since
    Aug. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    185111-plasmid-map-sequence-id-368105

    Enlarge

  41. KBB611

    Plasmid
    #185112
    Purpose
    Pom152 central repeats 4 and 5 in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 repeats 4&5-6His
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185112-plasmid-map-sequence-id-368078

    Enlarge

  42. LDB223

    Plasmid
    #185428
    Purpose
    Bacterial expression Nup2 N-terminal domain as GST fusion
    Depositor
    Laura Davis, Ken Belanger
    Insert
    NUP2
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Nup2 N-terminal domain
    Promoter
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185428-plasmid-map-sequence-id-367563

    Enlarge

  43. KBB258

    Plasmid
    #185072
    Purpose
    Bacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusion
    Depositor
    Ken Belanger
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Yeast
    Mutation
    Nup1 N-term+FXFG repeats
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185072-plasmid-map-sequence-id-368132

    Enlarge

  44. pT7TS-rich

    Plasmid
    #99050
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.
    Depositor
    Richard BĂ©langer
    Type
    Empty backbone
    Use
    Unspecified; Xenopus oocyte system
    Tags
    Expression
    Mutation
    Promoter
    pT7
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    99050-plasmid-map-sequence-id-191760

    Enlarge

  45. pEGFP-N2-GRASP65

    Plasmid
    #137709
    Purpose
    Eukaryotic expression of rat GRASP65 full-length
    Depositor
    Yanzhuang Wang
    Insert
    GRASP65 (Gorasp1 Rat)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    137709-plasmid-map-sequence-id-273548

    Enlarge

  46. KBB96

    Plasmid
    #185113
    Purpose
    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated so only one FXFG repeat remains
    Depositor
    Ken Belanger
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Last FXFG domain of Nup1
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185113-plasmid-map-sequence-id-368072

    Enlarge

  47. KBB109

    Plasmid
    #185066
    Purpose
    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after NsiI restriction site
    Depositor
    Ken Belanger
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Nup1 C-terminal region, truncated at NsiI restric…
    Promoter
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    185066-plasmid-map-sequence-id-367178

    Enlarge

  48. HE_150

    Bacterial Strain
    #201051
    Purpose
    DH10B-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.
    Depositor
    Francis Nano
    Bacterial Resistance
    Streptomycin
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only

  49. HE_100

    Bacterial Strain
    #201050
    Purpose
    DH10B-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.
    Depositor
    Francis Nano
    Bacterial Resistance
    Streptomycin
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only

  50. HE_50

    Bacterial Strain
    #201049
    Purpose
    DH10B-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.
    Depositor
    Francis Nano
    Bacterial Resistance
    Streptomycin
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 3401 - 3450 of 49469 results