We narrowed to 14,561 results for: cas9 genes
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Plasmid#169857PurposeSauCas9 pegRNA for EMX1DepositorInsertSauCas9 EMX1-pegRNA
ExpressionMammalianPromoterhuman U6 promoterAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CASI-Cas9C
Plasmid#80932PurposeExpresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.DepositorInsertCas9C
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAaePUb::Cas9-2A-Neo
Plasmid#176680PurposeExpression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)DepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprExpressionInsectMutationDrosophila codon optimizedPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113343PurposeExpresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteinsDepositorInsertp300core-dCas9-VP64-T2A-MS2-p65-HSF1
UsePiggybacAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9GFP-W
Plasmid#200100PurposeLentiviral vector expressing Cas9 fused with GFP at the C-terminusDepositorInsertGFP
UseCRISPR and LentiviralAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
p221z-CAS9p-t35s
Plasmid#118385PurposeEntry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p
UseCRISPRAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Sa-SauriCas9
Plasmid#135967PurposeExpresses Sa-SauriCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVExpressionMammalianAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-NoCBE
Plasmid#209038PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)DepositorInsertnSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-BEACON2
Plasmid#209041PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLSDepositorInsertBEACON2-nSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-TadA8.20m
Plasmid#209045PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLSDepositorInsertTadA8.20m-nSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
miniABEMax V82G nSaCas9
Plasmid#135365PurposeExpression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitutionDepositorInsertminiABEMax V82G nSaCas9
UseCRISPRExpressionMammalianMutation(V82G)Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112924PurposepiggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UsePiggybac transposon vectorExpressionMammalianAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cp PS Intein dCas9-VPR
Plasmid#242031PurposeBlue-light-controlled release of dCas9-VPR from the cytoplasm to the nucleus.DepositorInsertCp PS Intein dCas9-VPR
ExpressionMammalianPromoterCMVAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330_TOMM20 sgRNA / hSpCas9
Plasmid#172836PurposeMammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-AsLOV2x3-NLSx3
Plasmid#231566PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
xCas9(3.6)-BE4
Plasmid#108386PurposeMammalian expression vector for xCas9(3.6)-BE4DepositorInsertxCas9(3.6)-BE4
TagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …Available SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gephyrin (px330:2-guideCas9)
Plasmid#169432PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRExpressionMammalianAvailable SinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D
Plasmid#110302PurposeExpresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
ExpressionMammalianAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only