We narrowed to 1,497 results for: MBP

1. pJ4M/TDP-43 S48E

   Plasmid

   #104481

   Purpose

   bacterial expression of TDP-43 S48E

   Depositor

   Nicolas Fawzi

   Article

   Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   S48E

   Promoter

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-hMICU1EF

   Plasmid

   #199180

   Purpose

   This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.

   Depositor

   Ming-Feng Tsai

   Article

   Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

   Insert

   MICU1 (MICU1 Human)
   

   Use

   Tags

   His6 and MBP, Thrombin site, TEV site

   Expression

   Mutation

   D421A, E432K, F433W, F453W

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a Cdk2ap1CAN-MutTER

   Plasmid

   #178033

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   Changed T(108)A, E(109)A, R(110)A

   Promoter

   T7LacI

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a Cdk2ap1ΔN (MT2B2)

   Plasmid

   #178034

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   First N-Terminal 27aa are deleted

   Promoter

   T7LacI

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMAL-PIAL2M-sim1

   Plasmid

   #67908

   Purpose

   Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

   Depositor

   Andreas Bachmair

   Article

   Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

   Insert

   PIAL2 (AT5G41580 Mustard Weed)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   E281-A496; VEDL 425-428 AAA

   Promoter

   P-lac

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

   Plasmid

   #111398

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

   Plasmid

   #111399

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT

   Plasmid

   #111400

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT

   Plasmid

   #111451

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-p160C

    Plasmid

    #46

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    expresses aa580-1330

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA-p160(1-200)

    Plasmid

    #43

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-p160(400-580)

    Plasmid

    #45

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    expresses only aa400-580

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA-p160(200-400)

    Plasmid

    #44

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Expresses only aa200-400

    Promoter

    Available since

    July 21, 2005

    Availability

    Academic Institutions and Nonprofits only

14. TFORF1269

    Plasmid

    #143482

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIVEP2 (HIVEP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF0751

    Plasmid

    #143729

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIVEP1 (HIVEP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRM053 His-TEV-Cas9 triNLS 0C

    Plasmid

    #196244

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRM049 His-TEV-Cas9 triNLS 2C

    Plasmid

    #196245

    Purpose

    Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TH0901-pOCC177-FUS-wt_opt(Nhe)

    Plasmid

    #221885

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TH0994-pOCC177-Fus-wt_(1-211)

    Plasmid

    #221886

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    PLD domain (aa 1-211)

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TH1148-pOCC177-FUS(Nhe)-PLD_YtoF

    Plasmid

    #221887

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    In PLD all Tyr (Y) changed to Phe (F)

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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