We narrowed to 12,294 results for: ache

1. pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

   Plasmid

   #194278

   Purpose

   Expresses gRNA and dSaCas9-KRAB from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

   Inserts

   humanized dSaCas9 KRAB T2A Thy1.1
   gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and hUbC

   Available since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEF5B-FRT-GFP-αTAT1

   Plasmid

   #27099

   Depositor

   Maxence Nachury

   Article

   Shida et al Proc Natl Acad Sci U S A. 2010 Nov 10. (

   Insert

   α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
   

   Use

   Tags

   GFP, S-tag, and TEV site

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRE-UBC-HLA-E SCT-T2A-GFP

   Plasmid

   #205461

   Purpose

   lentiviral plasmid for expression of HLA-E SCT

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   HLA-E SCT (HLA-E Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-Target-AIDmax

   Plasmid

   #139107

   Purpose

   Mammalian Target-AIDmax expression vector (pSI-626)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF5B-FRT-control-APEX

   Plasmid

   #73187

   Purpose

   Expresses "control"-APEX (cytosolic) in mammalian cells

   Depositor

   Maxence Nachury

   Article

   Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

   Insert

   control-APEX (Nphp3 Glycine max, Mouse)
   

   Use

   Tags

   internal GFP tag

   Expression

   Mammalian

   Mutation

   changed glycine 2 to alanine

   Promoter

   EF1alpha

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. N-BLa 1.2

   Plasmid

   #88999

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   N-BLa 1.2 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C-BLa 1.2

   Plasmid

   #89000

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   C-BLa 1.2 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-3xCTCF-TetO

   Plasmid

   #213023

   Purpose

   piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase

   Depositor

   Luca Giorgetti

   Article

   Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

   Insert

   3xCTCF-TetO
   

   Use

   Mouse Targeting; Piggybase integration (transposo…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. gRNA NIA TLS2

   Plasmid

   #196981

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs fused to TLS2 tags
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pU6-26, pU6-29

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. gRNA NIA TLS1

    Plasmid

    #196980

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS1 tags
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRE-UBC-HLA-G SCT-T2A-GFP

    Plasmid

    #205462

    Purpose

    lentiviral plasmid for expression of HLA-G SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    HLA-G SCT (HLA-G Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CaMKII-EGFPKASH

    Plasmid

    #124882

    Purpose

    labelling of neuronal nuclear envelope with EGFP

    Depositor

    Vassilis Pachnis

    Article

    Obata et al Nature. 2020 Feb;578(7794):284-289. doi:

    Insert

    EGFP
    

    Use

    AAV

    Tags

    KASH

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mTagBFP2-Farnesyl-5

    Plasmid

    #55295

    Purpose

    Localization: Plasma Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pEF5B-FRT-AP-Sstr3-GFP-DEST

    Plasmid

    #49098

    Purpose

    Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Sstr3 (Sstr3 Mouse)
    

    Use

    Tags

    GFP and acceptor peptide (AP)

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-h-mmPtch1-B-HA-TagBFP

    Plasmid

    #120910

    Purpose

    Expresses BFP tagged Ptch1-B

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-N-Ezrin

    Plasmid

    #202684

    Purpose

    fluorescent protein tagged ezrin

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    Mus musculus EZR(1-586):EGFP (Ezr Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF5B-FRT-AP-Smo-YFP-DEST

    Plasmid

    #49099

    Purpose

    Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Smo (Smo Mouse)
    

    Use

    Tags

    YFP and acceptor peptide (AP)

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)_Pmel-1_TCRB

    Plasmid

    #175288

    Purpose

    Expresses Pmel-1 TCR beta chain

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Suitable for ivt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)_Pmel-1_TCRA

    Plasmid

    #175287

    Purpose

    Expresses Pmel-1 TCRA alpha chain

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    T cell receptor alpha chain (Tcra Mouse)
    

    Use

    Suitable for ivt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge