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Showing: 3551 - 3600 of 17876 results

  1. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid
    #91819
    Purpose
    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
    Depositor
    Chris Hill
    Article
    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1
    Insert
    RPO21 (RPO21 Budding Yeast)
    Use
    Tags
    10xHis
    Expression
    Yeast
    Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP…
    Promoter
    Endogenous Rpb1 promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    91819-plasmid-map-sequence-id-176494

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  2. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid
    #159149
    Purpose
    expresses His-tagged HIV reverse transcriptase and untagged HIV protease
    Depositor
    Andrea Pauli
    Article
    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d
    Inserts
    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    HIV-1 group M/subtype B – BH10 strain
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159149-plasmid-map-sequence-id-310074

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  11. pZE21/UBP1/ClpS_V65I

    Plasmid
    #98567
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon
    Depositor
    George Church
    Article
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Contains the V65I mutation that increases discrim…
    Promoter
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98567-plasmid-map-sequence-id-203054

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  12. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

    Plasmid
    #91820
    Purpose
    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
    Depositor
    Chris Hill
    Article
    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1
    Insert
    RPO21 (RPO21 Budding Yeast)
    Use
    Tags
    10xHis
    Expression
    Yeast
    Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP…
    Promoter
    Endogenous Rpb1 promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    91820-plasmid-map-sequence-id-181105

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  13. pRS426-HsFECH

    Plasmid
    #174524
    Purpose
    Expresses human ferrochelatase (FECH) in yeast
    Depositor
    Oleh Khalimonchuk
    Article
    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.
    Insert
    FECH with yeast HEM15 promoter and terminator (FECH Budding Yeast, Synthetic, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Lacks original mitochondrial targeting sequence (…
    Promoter
    HEM15 (yeast, 257 bp)
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    174524-plasmid-map-sequence-id-342886

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  14. pCag SE (Self-excising) FlpO-2A-Cre EV

    Plasmid
    #130986
    Purpose
    episomal expression of FlpO and Cre recombinases, self excising
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    FlpO-2A-Cre
    Use
    Cre/Lox and Unspecified; Episomal expression vect…
    Tags
    Expression
    Mammalian
    Mutation
    protamine intron added to FlpO to prevent bacteri…
    Promoter
    CMV/Chick β-actin (CAG)
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    130986-plasmid-map-sequence-id-304997

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  15. pJOP-HTT-HR65Q

    Plasmid
    #92249
    Purpose
    HTT donor DNA with 65Q for homologous recombination-mediated gene modification
    Depositor
    Mahmoud Pouladi
    Article
    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7
    Insert
    HTT donor DNA (HTT Human)
    Use
    Precisionx hr targeting vector
    Tags
    Expression
    Mutation
    Barcode of wobble bases to prevent TALEN cleaving…
    Promoter
    Available since
    Feb. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    92249-plasmid-map-sequence-id-182406

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  16. pJOP-HTT-HR45Q

    Plasmid
    #92248
    Purpose
    HTT donor DNA with 45Q for homologous recombination-mediated gene modification
    Depositor
    Mahmoud Pouladi
    Article
    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7
    Insert
    HTT donor DNA (HTT Human)
    Use
    Precisionx hr targeting vector
    Tags
    Expression
    Mutation
    Barcode of wobble bases to prevent TALEN cleaving…
    Promoter
    Available since
    Feb. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    92248-plasmid-map-sequence-id-182404

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  17. pJOP-HTT-HR81Q

    Plasmid
    #92250
    Purpose
    HTT donor DNA with 81Q for homologous recombination-mediated gene modification
    Depositor
    Mahmoud Pouladi
    Article
    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7
    Insert
    HTT donor DNA (HTT Human)
    Use
    Precisionx hr targeting vector
    Tags
    Expression
    Mutation
    Barcode of wobble bases to prevent TALEN cleaving…
    Promoter
    Available since
    Feb. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    92250-plasmid-map-sequence-id-315766

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  18. pJOP-HTT-HR30Q

    Plasmid
    #92247
    Purpose
    HTT donor DNA with 30Q for homologous recombination-mediated gene modification
    Depositor
    Mahmoud Pouladi
    Article
    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7
    Insert
    HTT donor DNA (HTT Human)
    Use
    Precisionx hr targeting vector
    Tags
    Expression
    Mutation
    Barcode of wobble bases to prevent TALEN cleaving…
    Promoter
    Available since
    Feb. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    92247-plasmid-map-sequence-id-182400

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  19. pUC57-BBclone1

    Plasmid
    #137069
    Purpose
    E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Inserts
    BB0405 (BB_0405 Borrelia burgdorferi B31)
    BB0406
    P66
    BBA57
    HtrA
    RevA (revA Borrelia burgdorferi B31)
    BB0238
    Use
    Tags
    Expression
    Bacterial
    Mutation
    wild-type, full-length (signal-peptide-containing…
    Promoter
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137069-plasmid-map-sequence-id-275695

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  21. pJH143

    Plasmid
    #68382
    Purpose
    reverse IMCE donor plasmid
    Depositor
    Trevor Charles
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pET29b-Cwc25

    Plasmid
    #111341
    Purpose
    his tagged Cwc25
    Depositor
    Christine Guthrie
    Insert
    CWC25
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    111341-plasmid-map-sequence-id-217724

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  23. pET28B-Cwc25

    Plasmid
    #111340
    Purpose
    his tagged Cwc25
    Depositor
    Christine Guthrie
    Insert
    CWC25
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    111340-plasmid-map-sequence-id-217722

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  24. pOME0294M

    Plasmid
    #182623
    Purpose
    KSHV anti-RTA_3Kb_Reverse
    Depositor
    Dirk Dittmer
    Insert
    KSHV anti-RTA_3Kb_Reverse
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion at 2853
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    182623-plasmid-map-sequence-id-367109

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  25. pRS316-Cwc25

    Plasmid
    #111343
    Purpose
    Cwc25 in pRS316(Ura) vector
    Depositor
    Christine Guthrie
    Insert
    CWC25
    Use
    Tags
    none
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    111343-plasmid-map-sequence-id-217945

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  26. pYBS187

    Plasmid
    #18901
    Depositor
    Elaine Elion
    Insert
    Cdc28 (CDC28 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 27, 2008
    Availability
    Academic Institutions and Nonprofits only

  27. pAG25-1199 cDNA

    Plasmid
    #160379
    Purpose
    N1199 narnavirus cDNA
    Depositor
    John McCusker
    Insert
    N1199
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    NA
    Available since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    160379-plasmid-map-sequence-id-314410

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  28. pOME0292M

    Plasmid
    #182621
    Purpose
    PAN-RNA_RevHuman herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
    Depositor
    Dirk Dittmer
    Insert
    Pan_RNA_Rev
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182621-plasmid-map-sequence-id-359446

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  29. pGEX-Cwc25-GST

    Plasmid
    #111339
    Purpose
    Cwc25 with C-term GST tag
    Depositor
    Christine Guthrie
    Insert
    CWC25
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111339-plasmid-map-sequence-id-214708

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  30. pRS314-URA3

    Plasmid
    #11004
    Depositor
    Francisco Malagon
    Insert
    URA3 (URA3 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Promoter
    Available since
    April 28, 2006
    Availability
    Academic Institutions and Nonprofits only
    11004-plasmid-map-sequence-id-6838

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  31. RPL25-GFP (94)

    Plasmid
    #24037
    Depositor
    Michael Rout
    Insert
    RPL25 (RPL25 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 10, 2010
    Availability
    Academic Institutions and Nonprofits only

  32. YEplac181-BUD32

    Plasmid
    #10997
    Depositor
    Francisco Malagon
    Insert
    BUD32 (BUD32 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Promoter
    Available since
    Dec. 16, 2005
    Availability
    Academic Institutions and Nonprofits only
    10997-plasmid-map-sequence-id-6832

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  33. pNRB65

    Plasmid
    #36455
    Depositor
    Douglas Bishop
    Insert
    ARS501-LEU2 (LEU2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    T7
    Available since
    Aug. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    36455-plasmid-map-sequence-id-45658

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  34. pRS415-RSC2

    Plasmid
    #11001
    Depositor
    Francisco Malagon
    Insert
    RSC2 (RSC2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Promoter
    Available since
    Dec. 29, 2005
    Availability
    Academic Institutions and Nonprofits only
    11001-plasmid-map-sequence-id-6835

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  35. YEplac181-NAR1

    Plasmid
    #10999
    Depositor
    Francisco Malagon
    Insert
    NAR1 (NAR1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Promoter
    Available since
    Dec. 16, 2005
    Availability
    Academic Institutions and Nonprofits only
    10999-plasmid-map-sequence-id-6833

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  36. Sc PCNA-pET(11a)

    Plasmid
    #239197
    Purpose
    Overexpress Sc PCNA in E. coli
    Depositor
    Michael O'Donnell
    Insert
    PCNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239197-plasmid-map-sequence-id-474410

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  37. pOME0299M

    Plasmid
    #182627
    Purpose
    Reverse T61 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
    Depositor
    Dirk Dittmer
    Insert
    T61_Reverse
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182627-plasmid-map-sequence-id-359429

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  38. YEplac181-RSC2

    Plasmid
    #11000
    Depositor
    Francisco Malagon
    Insert
    RSC2 (RSC2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Promoter
    Available since
    Dec. 16, 2005
    Availability
    Academic Institutions and Nonprofits only
    11000-plasmid-map-sequence-id-6834

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  39. pPS3858

    Plasmid
    #113393
    Purpose
    galactose-inducible Ty3-his3AI; additional PPT
    Depositor
    Suzanne Sandmeyer
    Insert
    Ty3-his3AI
    Use
    Tags
    his3AI
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    113393-plasmid-map-sequence-id-218651

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  40. pGEX-Slu7

    Plasmid
    #111352
    Purpose
    protein purification
    Depositor
    Christine Guthrie
    Insert
    SLU7
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111352-plasmid-map-sequence-id-217210

    Enlarge

  41. pPS293

    Plasmid
    #8851
    Depositor
    Pamela Silver
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 3, 2005
    Availability
    Academic Institutions and Nonprofits only

  42. pGal80,y+

    Plasmid
    #26851
    Depositor
    Christopher Potter
    Insert
    Gal80 (GAL80 Budding Yeast)
    Use
    Tags
    Yellow Gene (Drosophila)
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 22, 2010
    Availability
    Academic Institutions and Nonprofits only
    26851-plasmid-map-sequence-id-12719

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  43. RPS1B-GFP (95)

    Plasmid
    #24039
    Depositor
    Michael Rout
    Insert
    RPS1B (RPS1B Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 31, 2010
    Availability
    Academic Institutions and Nonprofits only

  44. pRS415-BUD32

    Plasmid
    #10991
    Depositor
    Francisco Malagon
    Insert
    BUD32 (BUD32 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Promoter
    Available since
    Dec. 29, 2005
    Availability
    Academic Institutions and Nonprofits only
    10991-plasmid-map-sequence-id-220924

    Enlarge

  45. pRS415-NAR1

    Plasmid
    #10995
    Depositor
    Francisco Malagon
    Insert
    NAR1 (NAR1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Promoter
    Available since
    Dec. 29, 2005
    Availability
    Academic Institutions and Nonprofits only
    10995-plasmid-map-sequence-id-273251

    Enlarge

  46. pRS314-Cef1-M175I

    Plasmid
    #234118
    Purpose
    Suppressor screen mutant in Cef2
    Depositor
    Aaron Hoskins
    Insert
    Cef1
    Use
    Tags
    Expression
    Yeast
    Mutation
    M175I
    Promoter
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234118-plasmid-map-sequence-id-466510

    Enlarge

  47. pRS314-Cef1-Q193P

    Plasmid
    #234117
    Purpose
    Suppressor screen mutant in Cef1
    Depositor
    Aaron Hoskins
    Insert
    Cef1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Q193P
    Promoter
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234117-plasmid-map-sequence-id-466508

    Enlarge

  48. pRS314-Cef1-A37V

    Plasmid
    #234119
    Purpose
    Suppressor screen mutant in Cef3
    Depositor
    Aaron Hoskins
    Insert
    Cef1
    Use
    Tags
    Expression
    Yeast
    Mutation
    A37V
    Promoter
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234119-plasmid-map-sequence-id-466463

    Enlarge

  49. Stag-ObMT

    Plasmid
    #215161
    Purpose
    Shanbhag lab plasmids
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    Stag-ObMT
    Use
    Tags
    S-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215161-plasmid-map-sequence-id-430154

    Enlarge

  50. pOME0300M

    Plasmid
    #182628
    Purpose
    Reverse sequence of anti-PAN Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.
    Depositor
    Dirk Dittmer
    Insert
    antiPAN_Rev
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182628-plasmid-map-sequence-id-359478

    Enlarge

Showing: 3551 - 3600 of 17876 results