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Plasmid#105309PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159/666APromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S666A
Plasmid#105308PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS666APromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP KInd2 S159A
Plasmid#105306PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159APromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-LIN-29-pcDNA3.1-
Plasmid#52515Purposeexpresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertLin-29 b (lin-29 Nematode)
UseTagsRGS-6xHisExpressionMammalianMutationwt (changed coding sequence in 5' (5th codon…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIG-FLAG-MLL-AF9
Plasmid#71443PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFPDepositorInsertMLL-Af9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIR-FLAG-MLL-AF9
Plasmid#71444PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2DepositorInsertMLL-AF9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
PEmax_AAVS1_knockin_HDR_donor
Plasmid#223000PurposeHDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPEmax
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-eGFP-Flag-SETX
Plasmid#218838PurposeMammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.DepositorInsertSenataxin (SETX Human)
UseTagsFlag and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHmScarlet
Plasmid#185538PurposeExpresses LGI1-pHmScarlet under the CamKII promoterDepositorInsertLGI1 (Lgi1 Rat)
UseLentiviralTagspHmScarletExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NST1_pOpOn2.1
Plasmid#228065PurposeExpresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.DepositorInsertNAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
UseDexamethasone inducible vectorTagsExpressionPlantMutationPromoterCaMV35S for LhGR and pOp6-minimal CaMV35S for NST1Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOD2046-bwmDEG
Plasmid#89367PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPmyo-3Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
α‐eGFP
Plasmid#160214PurposeRetroviral construct to express an eGFP-tagged version of the α subunit of AP2DepositorUseRetroviralTagseGFP at Hinge regionExpressionMutationBrain-specific InsertPromoterAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPACE-NG (pRZ5150)
Plasmid#140244PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE_NG
UseTagsExpressionMammalianMutationV82G in TadA*, NG mutations in SpCas9, D10A in Sp…PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
miABE
Plasmid#205412PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
UseTagsExpressionMammalianMutationD61A+E85R+H369R+S387R+S457RPromoterhU6, CAG, CMVAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424TagsExpressionMutationThe conserved GDD in the NS5 polymerase gene muta…PromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHluorin
Plasmid#185537PurposeExpresses LGI1-pHluorin under the CamKII promoterDepositorInsertLGI1 (Lgi1 Rat)
UseLentiviralTagspHluorinExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPACE (pRZ1813)
Plasmid#140242PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE
UseTagsExpressionMammalianMutationV82G in TadA*, D10A in Cas9, R187W in pmCDA1PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOD2048-csnDEG
Plasmid#89368PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPosm-6Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPACE-VRQR (pRZ5137)
Plasmid#140245PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE_VRQR
UseTagsExpressionMammalianMutationV82G in TadA*, VRQR mutations in SpCas9, D10A in …PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY2clust-mCherry-RANK-P2A-CIBNcaax
Plasmid#208613PurposeMammalian expression of CRY2clust-mCherry-RANK-P2A-CIBNcaax (Opto-RANKm)DepositorInsertCRY2clust-mCherry-RANK-P2A-CIBNcaax (Cry2 Mouse, Mustard Weed)
UseTagsCAAX and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only