Addgene

We narrowed to 12,617 results for: NUC

Showing: 1421 - 1440 of 12617 results

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pTriEx-mCherry::LANS4

Plasmid

#60785

Purpose

Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

Depositor

Brian Kuhlman

Article

Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

Insert

LANS4

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

Plasmid

#86413

Purpose

nuclear split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

Nop1pr-GFP11-mCherry-PUS1

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H2A-Akt-STOPS

Plasmid

#175010

Purpose

Akt-STOPS targeted to the nucleus via histone H2A.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

H2A-mCherry-Akt-STOPS

Use

Tags

Histone H2A and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046785

Plasmid

#145684

Purpose

Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1-KSENB-Nup133

Plasmid

#87340

Purpose

To express human Nup133 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion of Nup133 AA1-7

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDB22

Plasmid

#61342

Purpose

Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Use

Tags

GS linker-AsLov2-cMyc1A NLS and PKIt NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-PmCDA1

Plasmid

#79615

Purpose

Expresses dCas9-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

PmCDA1

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR2-NES

Plasmid

#175011

Purpose

Cytosol-targeted AktAR2.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

AktAR-NES

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJK511

Plasmid

#155384

Purpose

dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pTetOne-DsRed-cNLS

Plasmid

#138528

Purpose

Inducible expression of DsRed-Express2 with nuclear localization signal

Depositor

Ana Teixeira, Christopher Grigsby

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

DsRed-Express2

Use

Tags

3x SV40 cNLS

Expression

Mammalian

Mutation

Promoter

TRE3Gs

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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Tmem214 pmRFP-N2 (1348)

Plasmid

#62047

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
p1553 CMV-EBNA1 (delta GGA)

Plasmid

#26648

Depositor

Bill Sugden

Article

Aiyar et al J Biol Chem. 1998 Dec 4. 273(49):33073-8

Insert

Epstein-Barr Nuclear Antigen 1 (EBNA-1 Epstein-Barr Virus)

Use

Tags

Expression

Mammalian

Mutation

deletion of 224 amino acids of EBNA-1 (aa101-324 …

Promoter

Available since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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WN10151

Plasmid

#80441

Purpose

Expresses human codon-optimized dead (832A) LbCpf1

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Insert

dead (D832A) LbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

D832A

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

Plasmid

#86414

Purpose

nuclear split GFP11 reporter without mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP11-PUS1

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1pr

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDN42

Plasmid

#61345

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Use

Tags

GS linker-AsLov2-biNLS10 and PKIt NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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Tmem214 pEGFP-N2 (1072)

Plasmid

#62026

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro

Plasmid

#170467

Purpose

The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

Depositor

Raphael Gaudin

Article

Bakhache et al bioRxiv 2021

Insert

Nucleocapsid

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry::LANS1

Plasmid

#60784

Purpose

Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

Depositor

Brian Kuhlman

Article

Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

Insert

LANS1

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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RFN_EGFP_81

Plasmid

#60073

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only
SL2-sgTelo-MTSa/BFP/pdCas9-C1

Plasmid

#162760

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Use

Tags

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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