We narrowed to 3,058 results for: PPL

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pCDFBB-eGFP

Plasmid

#32550

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

egfp (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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px330 VRER

Plasmid

#101729

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA iRFP713_zhang2.0

Plasmid

#167916

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-mVenus-dspB(E184Q)-6xHis

Plasmid

#176576

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Hexahistidine tag and mVenus

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA EBFP2

Plasmid

#167905

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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p458 VRER

Plasmid

#101728

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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p330 VQR

Plasmid

#101730

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Puro

Plasmid

#167871

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDI

Plasmid

#60555

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDI

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC-eGFP

Plasmid

#107408

Purpose

This plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV Syn intron Psam4GlyR m3m4 HA

Plasmid

#196041

Purpose

AAV for Chemogenetic plasmids

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

Psam4GlyR m3m4 HA

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

Synapsin

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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mTFP1-CathespinB-6

Plasmid

#55467

Purpose

Localization: Lysosomes, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

CathespinB

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pDEST-hPDIb′a′

Plasmid

#60556

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDIb'a' tag

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_KanR neo

Plasmid

#167861

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_EBFP2

Plasmid

#167857

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUCBB-ptet-eGFP

Plasmid

#32554

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

ptet

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pST-KNarK2

Plasmid

#44565

Purpose

for ectopic hypoxia inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pUCBB-pBAD-eGFP

Plasmid

#32553

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

pBAD

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1F74G-FLAG

Plasmid

#189727

Purpose

Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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