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pLVX-EF1a-PTS1-IRES-Puromycin

Plasmid

#134665

Purpose

Expresses EGFP-tagged peroxisome marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

tripeptide SKL (Peroxisome targeting signal 1)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCEP4-myc-HLA-A24

Plasmid

#135507

Purpose

Mammalian expression of myc-tagged HLA-A*024:02

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*24:02 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6011nes

Plasmid

#108652

Purpose

An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Publication

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hyBRET-ERK-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2

Plasmid

#112004

Purpose

For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2

Depositor

Lin Tian

Publication

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has service

AAV9

Insert

axon-GCaMP6s-P2A-mKate2

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMito-LSSmOrange

Plasmid

#37135

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LSSmOrange

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pTorPE-GEM-GECO1

Plasmid

#32463

Depositor

Robert Campbell

Publication

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEM-GECO1

Use

Lab constructed

Tags

6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCX459

Plasmid

#229214

Purpose

CRISPR array plasmid for MAP1B (Spacers 1-24)

Depositor

Jennifer Doudna

Publication

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRISPR array plasmid for MAP1B (Spacers 1-24)

Expression

Mammalian

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pVQ CMV NanoV1Mutant-2a-EGFP ferritin

Plasmid

#79650

Purpose

Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein

Depositor

Jeffrey Friedman

Publication

Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

Inserts

transient receptor potential vanilloid 1 (Trpv1 Rat)
camelid anti-GFP nanobody
Enhanced green fluorescent protein
mouse ferritin light chain (Ftl1 Mouse)
mouse ferritin heavy chain (Fth1 Mouse)

Use

Adenoviral

Expression

Mammalian

Mutation

isoleucine 679 changed to lysine

Promoter

CMV

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe4_GCaMP6f

Plasmid

#213947

Purpose

AAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_GCaMP6f

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

GCaMP6f

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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Caspase-Reporter

Plasmid

#243673

Purpose

Fluorescent Reporter for Caspase 3/7 activity. Contains DEVD sequence.

Depositor

Matthias Wirth

Publication

Selcen et al Cell Death Discov. 2025 Aug 6;11(1):368.

Insert

Split-GFP with DEVD sequence and T2A mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(Hygro) TagBFP-hNucleolin

Plasmid

#182592

Purpose

expresses bfp-hNucleolin in mammalian cells

Depositor

Agnel Sfeir

Publication

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hNucleolin (NCL Human)

Tags

BFP

Expression

Mammalian

Promoter

CMV

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K13-15R

Plasmid

#63563

Purpose

Expression of human H2A with K13R and K15R tagged with Flag

Depositor

Titia Sixma

Publication

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 13 and Lysine 15 are now Arginine

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pHR-mCherry-tSH2(ZAP70)

Plasmid

#27137

Depositor

Ron Vale

Publication

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

mCherry and ZAP70 (residues 1-259) (ZAP70 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-WHaloCaMP1a-EGFP

Plasmid

#205313

Purpose

Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

Depositor

Eric Schreiter

Publication

Farrants et al Nat Methods. 2024 Oct;21(10):1916-1925.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WHaloCaMP1a-EGFP

Expression

Mammalian

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MXS_E2-Crimson

Plasmid

#62410

Purpose

MXS_chaining vector with E2-Crimson

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

E2-Crimson

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR2(T/A)

Plasmid

#161754

Purpose

Negative-control mutant for ExRai-AKAR2 biosensor.

Depositor

Jin Zhang

Publication

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ExRai-AKAR2(T/A)

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Beta1-GFP pFB-Neo

Plasmid

#69767

Purpose

Expresses Beta1-GFP integrin for retroviral transfection

Depositor

Martin Humphries

Publication

Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

Insert

Beta1 integrin (ITGB1 Human)

Use

Retroviral

Tags

GFP

Promoter

IRES

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pUC57-gCarvi-T2A

Plasmid

#190333

Purpose

Fluorescent reporter for cAMP

Depositor

Naoto Saitoh

Publication

Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gCarvi

Use

Unspecified

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ-mgU2-47-Mango II

Plasmid

#127586

Purpose

Expresses mgU2-47-Mango II scaRNA from a mU6 promoter

Depositor

David Rueda

Publication

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

mgU2-47 scaRNA with Mango II tag

Expression

Mammalian

Promoter

modified U6

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124431

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

Depositor

Xiaokun Shu

Publication

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Use

Zebrafish (if expression level is too high in zeb…

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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