165,637 results
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Plasmid#172565PurposeInducible gene expression, pACYC origin, CymRAM repressor, PCymRC promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertCymRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pQLink-GST-4xCTD
Plasmid#138471PurposeExpresses GST-tagged 4xCTD heptapeptide repeat in bacteriaDepositorInsertRpb1 (POLR2A Human)
UseTagsGSTExpressionBacterialMutation4x CTD repeatPromoterT5Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH79
Plasmid#127704PurposePttr-39::gfp1-10DepositorInsertGFP1-10
UseTagsExpressionWormMutationPromoterttr-39Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH86
Plasmid#127706PurposePmyo-3::GFP1-10DepositorInsertGFP1-10
UseTagsExpressionWormMutationPromotermyo-3Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ14902
Plasmid#239277PurposeExpresses gG2 for pertubing endogenous human GAPDH mRNA via CRISPR-TODepositorInsertPspCas13b-hGAPDH-gRNA2 (GAPDH Human)
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10622
Plasmid#239274PurposeExpresses PYL1-KIFC1* for the retrograde transport of RNA along microtubules via CRISPR-TODepositorInsertPYL1-BFP-KIFC1(aa125-673) (KIFC1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10550
Plasmid#239269PurposeExpresses PYL1-MAVS* for recruiting RNA to the outer mitochondrial membrane via CRISPR-TODepositorInsertPYL1-BFP-MAVS(aa510-540) (MAVS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10552
Plasmid#239271PurposeExpresses PYL1-G3BP1 for recruiting RNA to the stress granule via CRISPR-TODepositorInsertPYL1-BFP-G3BP1 (G3BP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEMTB-mScarlet3-H (aka mYongHong)
Plasmid#239407PurposeExpresses EMTB-mScarlet3-H in mammalian cellsDepositorInsertEMTB (MAP7 Human)
UseTagsmScarlet3-H (aka mYongHong)ExpressionMammalianMutationM163H in mScarlet3PromoterCMVAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTom20-mScarlet3-H (aka mYongHong)
Plasmid#239412PurposeExpresses Tom20-mScarlet3-H in mammalian cellsDepositorInsertTom20 (TOMM20 Human)
UseTagsmScarlet3-H (aka mYongHong)ExpressionMammalianMutationM163H in mScarlet3PromoterCMVAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNEU-KlacRS
Plasmid#232155PurposeSite-specific incorporation of L-lactyl-lysine into target proteins in mammalian cells.DepositorInsertKlacRS1
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mCherry-TAG-EGFP
Plasmid#232156PurposeA fluorescence reporter to show incorporation of target unnatural amino acids.DepositorInsertmCherry-linker(TAG)-EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MmKlacRS1
Plasmid#232157PurposeSite-specific incorporation of L-lactyl-lysine into target proteins in E. coli cells.DepositorInsertKlacRS1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR_sfGFP-TurboID
Plasmid#240226PurposeGateway entry clone with TurboID tagged with superfolder GFP (contains stop codon)DepositorInsertsfGFP-TurboID
UseGateway shuttle vectorTagsExpressionMutationPromoterAvailable sinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pStayGold-Tubulin
Plasmid#229571PurposeExpresses StayGold-tubulin fusion in mammalian cellsDepositorInsertStayGold
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-EGFP
Plasmid#215537PurposeGateway entry vector with the EGFP fluorescent proteinDepositorInsertEGFP
UseGateway entry vectorTagsExpressionMutationPromoternoneAvailable sinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
FLuc-N-HiBiT
Plasmid#207117PurposeBacterially expressed HiBiT-FLuc for Ni-NTA purificationDepositorInsertluciferin 4-monooxygenase
UseTagsHiBiT Tag and His-tag with thrombin siteExpressionBacterialMutationCodon optmization for expression in BL21DE3PromoterT7 PromotorAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBIG1e-CDK12deltaRS/Cyclin K
Plasmid#231744PurposeProtein expression in insect cellsDepositorUseTagsFlag-3CExpressionInsectMutationCDK12(delta104-380)PromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS_SpyCatcher:mKate2
Plasmid#225188PurposeUsed for inserting SpyCatcher fused to mKate2 at the bacterial Tn7 siteDepositorInsertSpyCatcher:mKate2
UseTagsSpyCatcherExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH61 pLIB-8His3C-spt4
Plasmid#231649Purposeexpress S.cerevisiae spt4 in insect cellsDepositorInsertspt4 (SPT4 Budding Yeast)
UseTags8His-HRV3CExpressionInsectMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH60 pLIB-spt5
Plasmid#231648Purposeexpress S.cerevisiae spt5 in insect cellsDepositorInsertspt5 (SPT5 Budding Yeast)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH1 Dual G-less cassette
Plasmid#231645Purposeplasmid containing dual G-less cassette for in vitro transcriptionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-hGeminin
Plasmid#199344Purposemodified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human GemininDepositorInsertATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
UseTagscas9 c-term fused to hgemenin 1-110 fragmentExpressionMammalianMutationcas9 c-term fused to hgemenin 1-110 fragmentPromotercbhAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCN34e-yfp
Plasmid#227641PurposeNegative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertyfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCN34e-kimA-yfp
Plasmid#227638Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertkimA-yfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-NRG1-ScNeo
Plasmid#209906PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertNRG1-ScNeo (NRG1 Human)
UseTagsmNeonGreen and mScarletExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH2-ubi-Y-pyrG
Plasmid#221131PurposeExpression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …TagsExpressionMutationPromoterPalcA-T1-PalcS/M-T2-PaldA expression cassette (al…Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH2-ubi-M-pyrG
Plasmid#221132PurposeExpression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …TagsExpressionMutationPromoterPalcA-T1-PalcS/M-T2-PaldA expression cassette (al…Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG eGFP NLS
Plasmid#170830PurposeDonor plasmid for knocking eGFP NLS into AAVS1 safe harbor locusDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALENTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only