We narrowed to 22,630 results for: CRISPR

1. XClone_Cas9_KRAB_T2A_BFP

   Plasmid

   #141208

   Purpose

   Tunable and temporal expression control of dCas9

   Depositor

   Rory Johnson

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFETCh_ZNF3

   Plasmid

   #72362

   Purpose

   Donor vector for 3' FLAG tag of human ZNF3

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   ZNF3 Homology Arms (ZNF3 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

   Plasmid

   #221537

   Purpose

   Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain

   Depositor

   Omar Akbari

   Insert

   DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1203T-CMV-Cas7.1-NLS-bGHpolyA

   Plasmid

   #221540

   Purpose

   Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1203W-CMV-Cas7.1-7.2-bGHpolyA

   Plasmid

   #220961

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and Cas7.2 domains from DiCas7-11, deactivated (truncated from DiCas7-11 effector)

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pR6K-GFP-CASTIB

   Plasmid

   #199655

   Purpose

   R6K plasmid with a I-B CAST but no CRISPR array

   Depositor

   IIya Finkelstein

   Insert

   CAST I-B systems
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR6K-GFP-CASTV

   Plasmid

   #199657

   Purpose

   R6K plasmid encoding a type V CAST but no CRISPR array

   Depositor

   IIya Finkelstein

   Insert

   CAST V systems
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXZ13lacZalphaKanR

   Plasmid

   #160230

   Purpose

   Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.

   Depositor

   Ian Duncan

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRGEB32

   Plasmid

   #63142

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEcCas

    Plasmid

    #73227

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; Red recombinase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native promoter

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBC-DT1T2

    Plasmid

    #50590

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, U626t plus, U629p, T2
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCBC-DT3T4

    Plasmid

    #50592

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T3, gRNA scaffold, U6-1tplus, U6-26p, T4
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCBC-DT2T3

    Plasmid

    #50591

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSGKP-km

    Plasmid

    #117233

    Purpose

    A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. px330-mcherry

    Plasmid

    #98750

    Purpose

    Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

    Depositor

    Jinsong Li

    Article

    Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCas9n(D10A)-Blast

    Plasmid

    #63593

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, nickase mutant

    Promoter

    EFS-NS

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Dm-snRNA:U6:96Ab

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPUR-hU6-sgRNA-Sirius-8XMS2

    Plasmid

    #121942

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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