Addgene

We narrowed to 5,630 results for: chia

Showing: 1461 - 1480 of 5630 results

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pBAD-I-SceI

Plasmid

#60960

Purpose

Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

I-SceI restriction enzyme

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJMK001

Plasmid

#33780

Depositor

Adam Cohen

Article

Kralj et al Science. 2011 Jul 15;333(6040):345-8.

Insert

Proteorhodopsin Optical Proton Sensor

Use

Tags

Expression

Bacterial

Mutation

D97N

Promoter

AraBAD

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pKB232

Plasmid

#170019

Purpose

Encodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CRAMP

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBSK

Plasmid

#67504

Purpose

Express the tRNALys scaffold

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys human

Use

Tags

Expression

Bacterial

Mutation

Promoter

lpp

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pOPH6

Plasmid

#40315

Depositor

Shaun Lott

Article

Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

Insert

Peptide:N-glycosidase F

Use

Tags

6xHis and OmpA3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pLRD11

Plasmid

#208263

Purpose

Production of sclareol in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
abCAS
ssSS

Use

Tags

Expression

Bacterial

Mutation

abCAS(D537A)

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD14

Plasmid

#208266

Purpose

Production of syn-pimara-7,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD17

Plasmid

#208269

Purpose

Production of syn-stemod-13(17)-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBbE1k-DDC

Plasmid

#71723

Purpose

dopa decarboxylase from pig

Depositor

Taek Soon Lee

Article

Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

Insert

dopa decarboxylase

Use

Tags

Expression

Mutation

Promoter

pTrc

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pSG 25

Plasmid

#63867

Purpose

Expression of beta-galactosidase at high levels in E. coli

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
MazF-WT

Plasmid

#155202

Purpose

To express recombinant MazF protein.

Depositor

Guanzheng Luo

Article

Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

Insert

MazF

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pKB231

Plasmid

#170018

Purpose

Encodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Cecropin P1

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pADS

Plasmid

#19040

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

codon-optimized synthetic amorphadiene synthase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pMYO

Plasmid

#34626

Depositor

Dieter Söll, Jesse Rinehart

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

Myoglobin

Use

Tags

6xHIS

Expression

Bacterial

Mutation

codon-optimized

Promoter

lpp

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBSKrnaSeph

Plasmid

#67506

Purpose

Express the tRNALys scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys with sephadex aptamer

Use

Tags

Expression

Bacterial

Mutation

Promoter

lpp

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pBSMrnaSeph

Plasmid

#67507

Purpose

Express the tRNAMet scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNAMet with sephadex aptamer

Use

Tags

Expression

Bacterial

Mutation

Promoter

lpp

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEZY19

Plasmid

#18668

Purpose

pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Gateway destination vector

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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SJZ7-KAN

Plasmid

#228260

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

kan

Use

Tags

Expression

Yeast

Mutation

Promoter

GPM1 S.c.

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AC

Plasmid

#108686

Purpose

Plasmid for assembly of donor DNA templates consisting of two expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AD

Plasmid

#108687

Purpose

Plasmid for assembly of donor DNA templates consisting of three expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge