-
Plasmid#50799PurposeSuicide vector for generating chromosomal deletions in multidrug-resistant bacteriaDepositorInserttellurite-resistance cassette
UseSuicide vectorTagsExpressionMutationPromoterPCs12Available sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Membrane proteins
Pooled Library#1000000124PurposeCRISPR gRNA mouse knockout pooled library - Membrane proteinsDepositorAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Ndi-1/myc-His
Plasmid#127503PurposeExpresses myc-tagged yeast Ndi-1 in mammalian cellsDepositorInsertNdi-1 (NDI1 Budding Yeast)
UseTagsmyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133B
Plasmid#69283PurposeGolden Gate entry vector; 3rd gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-dLight1.3b (AAV1)
Viral Prep#125560-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
UbC-blasticidine-P2A-TETon-3G transactivator
Plasmid#200191PurposeA TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertblasticidine - P2A - TETon-3G transactivator
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterUbCAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MPZ(P0)promoter pGL3-TATA
Plasmid#21259DepositorInsertmyelin protein zero promoter (Mpz Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV MaCPNS2)
Viral Prep#37825-MaCPNS2PurposeReady-to-use AAV MaCPNS2 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. These AAV were produced with the MaCPNS2 serotype, which permits efficient transduction of the PNS in rodents (including ENS in rats) and the CNS and PNS (including ENS) in marmosets and rhesus macaques. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
DiLiCre 2.0
Plasmid#198752PurposeA Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertCre-PhoCI-ERT2-ERT2
UseLentiviralTagsCre, kept in a photocleavable site, followed by 2…ExpressionBacterial and MammalianMutationPromoterTRE3GVAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgControl
Plasmid#125836PurposeKO controlDepositorInsertControl
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVS1-VIR2
Plasmid#134745PurposeVirulence and replication helperDepositorInsertvir gene cluster
UseVir gene expressionTagsExpressionMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpdCas9 KRAB
Plasmid#188499PurposeExpresses FLAG tagged SpdCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/H840APromoterEF1aAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)
Plasmid#209078PurposeNgn2 mediated cortical neuron induction, along with SNCA(3K) over-expressionDepositorUseTagssfGFPExpressionMammalianMutation3K mutant: E35K, E46K, E61KPromoterTRE4GAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
VGAT scFv [L118/14]
Plasmid#206754PurposeMammalian Expression of VGAT scFV. Derived from hybridoma L118/14 scFv.DepositorInsertVGAT (Mus musculus) recombinant scFV (Slc32a1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-DTR-GFP
Plasmid#124364Purposeconditional expression of a diphtheria toxin receptor (DTR)–GFP fusion proteinDepositorHas ServiceAAV2InsertDTR-GFP
UseAAVTagsGFPExpressionMutationPromoterChicken B-actinAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mLemon
Plasmid#219399PurposeConstitutive expression of mLemonin E. coliDepositorInsertmLemon
UseTagsExpressionBacterialMutationPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1_Ec_rpoZ
Plasmid#128837PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.DepositorInsertrpoZ (rpoZ E. coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP- SEC61B
Plasmid#121159PurposeMammalian expression of GFP tagged SEC61B, used as a general ER markerDepositorInsertSEC61B (SEC61B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)
Plasmid#209080PurposeNgn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expressionDepositorUseTagssfGFPExpressionMammalianMutationA53TPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-TurboCas-NLS-Blast
Plasmid#232346PurposeLentiviral expression vector for dCas9 fused to miniTurboNLSDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA, miniTurbo, V5 and 3xSV40 NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only