We narrowed to 18,609 results for: Met

1. pCCL-PGK-SPdCas9-BFP-EGFP

   Plasmid

   #66821

   Purpose

   dCas9 fused to BFP and EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-EGFP
   

   Use

   Tags

   dSpCas9-BFP-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3b(1454/3172)P2P-wt

   Plasmid

   #21404

   Depositor

   Eric Metzen

   Article

   Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pdest dTomato Puro

   Plasmid

   #223054

   Purpose

   dTomato gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   dTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. rag2:CTDSP2_CE_pISceI

   Plasmid

   #223033

   Purpose

   Express CTDSP2 gene in mesenchymal lineage of Zebrafish.

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   CTDSP2
   

   Use

   Express the insert(gene) in mesenchymal lineage o…

   Tags

   Expression

   Mutation

   Promoter

   Rag2

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRoC30_Th3.1

   Plasmid

   #188065

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.1)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 4 FuncLib mutations, described in publ…

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRoC30_Th3.7

   Plasmid

   #188066

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.7)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 3 FuncLib mutations, described in publ…

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJRoC30_Th3.10

   Plasmid

   #188067

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.10)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 3 FuncLib mutations, described in publ…

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSCrhaB2_ApyOAHIDS

   Plasmid

   #226246

   Purpose

   Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyD
   ApyO
   ApyH
   ApyI
   ApyS
   

   Use

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Synapsin-SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC

   Plasmid

   #213535

   Purpose

   Expresses the split transcription factors in cultured neuron and mouse brain

   Depositor

   Wenjing Wang

   Article

   Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

   Insert

   SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC-HA-HA
   

   Use

   AAV

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKG-GW2-NgBR

    Plasmid

    #203165

    Purpose

    Yeast expression vector for hNUS1

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    NUS1 (NUS1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. DNMT2Δ191-237

    Plasmid

    #198382

    Purpose

    Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

    Insert

    DNMT2 (TRDMT1 Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 191-237

    Promoter

    T7

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. p426-Cup1p-FusionRed

    Plasmid

    #188392

    Purpose

    Cu2+-dependent expression of red fluorescent protein in yeast cells

    Depositor

    Michihiko Kataoka

    Article

    Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

    Insert

    FusionRed
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1 promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuetG-HsDCP1a_539-582 (MCSII)_G

    Plasmid

    #146407

    Purpose

    Bacterial Expression of HsDCP1a_539-582

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    HsDCP1a_539-582 (DCP1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation T1716G compared to the sequen…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut2_G

    Plasmid

    #146409

    Purpose

    Bacterial Expression of HsDCP1a_539-582-mut2

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    HsDCP1a_539-582-mut2 (DCP1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation T1716G compared to the sequen…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSFDuetG-HsDCP1a_539-582 (MCSII)-WT_G

    Plasmid

    #146411

    Purpose

    Bacterial Expression of HsDCP1a_539-582

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    HsDCP1a_539-582 (DCP1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation T1716G compared to the sequen…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GA46

    Plasmid

    #196614

    Purpose

    Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    ATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGMC00007

    Plasmid

    #166725

    Purpose

    non targeting sgRNA

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    Non-targeting guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS313_ATP3G919C

    Plasmid

    #120255

    Purpose

    Expression of ATP3-7 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G919 changed to C; Alanine 307 changed to Proline

    Promoter

    ATP3

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS313_ATP3mut

    Plasmid

    #120256

    Purpose

    Expression of ATP3-67 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 304 changed to Asparagine; Alanine 307…

    Promoter

    ATP3

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCHKU33.1-2.2

    Plasmid

    #110666

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    PKS
    

    Use

    Tags

    flag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge