We narrowed to 9,269 results for: ef1

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VanR-wt

Plasmid

#153339

Purpose

Wild type VanR from Caulobacter crescentus. The gene is under the control of the TEF1 promoter

Depositor

Michael Jensen

Article

et al Biotechnology Notes, Volume 1, 2020, Pag

Insert

VanR

Expression

Yeast

Promoter

TEF1

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCEV-G1-Ph

Plasmid

#46814

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pCEV-G2-Km

Plasmid

#46815

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCEV-G2-Ph

Plasmid

#46816

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pLSPR31

Plasmid

#217316

Purpose

TEF1(p)-mRuby3:amdS fusion reporter

Depositor

James Fraser

Article

Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

Insert

Acetamidase

Tags

mRuby3

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCEV-G4-Ph

Plasmid

#46820

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and HXT7

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCEV-G3-Ph

Plasmid

#46818

Purpose

TEF1 and GAL10 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and GAL10

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pSSa23

Plasmid

#177373

Purpose

Expresses red-shifted luciferase under TEF1 promoter in Candida glabrata

Depositor

Dominique Sanglard

Article

Schrevens et al J Fungi (Basel). 2021 Oct 9;7(10). pii:

Insert

CgLUCopt-mut2

Promoter

TEF1

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-PM mir shHoxB9 b

Plasmid

#27020

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir HoxB9 3b

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pMYT026

Plasmid

#180679

Purpose

Encodes tTEF1 as a Type 4 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

tTEF1

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pIR3DH8

Plasmid

#165068

Purpose

Knocking out GAL80 using KlURA3 marker

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

gal80Arm1-P-AgTEF1-KlURA3-T-AgTEF1-gal80Arm2

Expression

Yeast

Mutation

WT

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-PM mir shHoxB9 a

Plasmid

#27021

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir HoxB9 2A

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pJW1511

Plasmid

#62365

Purpose

pEF1a-puro-T2A-ATHmmuRPL10A

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

Puro-T2A-AVITEVHAmmuRPL10A

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pJA291

Plasmid

#74487

Purpose

eF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFP

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

puroR-mCherry-T2A-eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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Aiolos AA130-189 in Artichoke

Plasmid

#74451

Purpose

Monitor post-translational degradation of Aiolos AA130-189 (Lentiviral, SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Insert

Aiolos (IKZF3) AA130-189 (IKZF3 Human)

Use

Lentiviral

Tags

EGFP

Promoter

SFFV and EF1a

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFPY2A

Plasmid

#165066

Purpose

Characterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFP

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>Y-FAST-ErB1.2A-yEGFP>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMV21

Plasmid

#128880

Purpose

Position 2 module- Tef1 promoter

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

Tef1 promoter

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCF738

Plasmid

#122032

Purpose

EF1a-WNV-protease-GFP

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0037

Plasmid

#181934

Purpose

Dual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

Gaussia luciferase
Cypridina luciferase

Expression

Mammalian

Promoter

CMV and EF1alpha

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDD426

Plasmid

#202079

Purpose

Frame +1/-0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD425

Plasmid

#202078

Purpose

Frame +0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +0 sgRNA

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD427

Plasmid

#202080

Purpose

Frame +2/-1 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYS19

Plasmid

#202084

Purpose

Frame -2 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame –2 sgRNA

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti_sgRNA(MS2)_zsGreen1

Plasmid

#138463

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-EGFP-hChibby1

Plasmid

#89770

Purpose

Labels mother centrioles/basal bodies

Depositor

Ken-Ichi Takemaru

Insert

Chibby1 (CBY1 Human)

Use

Lentiviral

Tags

EGFP

Promoter

EF1alpha

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pJZC533

Plasmid

#135435

Purpose

Yeast integrating plasmid for pTEF1-mCherry reporter gene

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

mCherry

Expression

Yeast

Mutation

codon optimized for S. cerevisiae

Promoter

pTEF1

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID8Z

Plasmid

#165053

Purpose

Integrative expression of yEGFP and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP5V1

Plasmid

#165058

Purpose

Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1- T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p413TEF-NYFP

Plasmid

#99557

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Microbiol. 2017 Apr;104(1):105-124.

Insert

Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP105

Plasmid

#165060

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP104

Plasmid

#165059

Purpose

Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID9L104

Plasmid

#165054

Purpose

Integrative expression of yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p413TEF-NQYFP

Plasmid

#99556

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Microbiol. 2017 Apr;104(1):105-124.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNA0306

Plasmid

#169452

Purpose

pRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmid

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

Cas9

Expression

Yeast

Promoter

TEF1p

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-G-Creon shRNA[Control]

Plasmid

#181824

Purpose

This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV and RNAi

Expression

Mammalian

Promoter

EF1a/U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ463

Plasmid

#187971

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ960

Plasmid

#187972

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ462

Plasmid

#187970

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ959

Plasmid

#192603

Purpose

TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

ScTEF1

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pABY-c45

Plasmid

#201771

Purpose

Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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