We narrowed to 2,475 results for: T2A

1. pPL001

   Plasmid

   #184750

   Purpose

   Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

   Depositor

   Jef Boeke

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Insert

   pEF1a-eGFP-T2A-BSD-bGHpA
   

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-Cas9-Blast

   Plasmid

   #83481

   Purpose

   Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

   Depositor

   Mohan Babu

   Inserts

   SpCas9
   Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   Tight Tre and human phosphoglycerate kinase 1 pro…

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP

   Plasmid

   #204723

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJA291

   Plasmid

   #74487

   Purpose

   eF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFP

   Depositor

   Andrew Fire

   Article

   Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

   Insert

   puroR-mCherry-T2A-eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHACK-QF2-AgU6-mCherry

   Plasmid

   #184530

   Purpose

   HACK-compatible plasmid for generating T2A-QF2 knockins in Anopheles mosquitoes with a mCherry selectable marker.

   Depositor

   Christopher Potter

   Article

   Raji et al Cell Rep. 2023 Feb 10;42(2):112101. doi:

   Insert

   QF2
   

   Use

   CRISPR

   Promoter

   Anopheles U6 promoter

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ac5-STABLE2-neo

   Plasmid

   #32426

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Insert

   Flag-mCherry-T2A-GFP-T2A-neo
   

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDT305

   Plasmid

   #128801

   Purpose

   Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone

   Depositor

   Bradley Bernstein

   Article

   Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

   Insert

   dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
   

   Use

   CRISPR and Lentiviral

   Promoter

   hUBC promoter

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TO-NFIA.1-SOX9 (bi-directional promoter)

   Plasmid

   #182306

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   NFIA (NFIA Human)
   SOX9 (SOX9 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Piggybac

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Promoter

   TRE3G-Bi-directional

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPGxRF3

   Plasmid

   #138384

   Purpose

   T2A-GAL4 knock-in cassette

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Insert

   T2A-GAL4
   

   Expression

   Insect

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TO-hNGN2-BSD-mApple

    Plasmid

    #182308

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    hNGN2 (NEUROG2 Human)
    bsd-T2A-mycNLS-mApple
    

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ6332 - ACTB CRISPRa CLIP Donor

    Plasmid

    #210015

    Purpose

    CLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    dLbCpf1-miniVPR-T2A-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBLO1808_arC9_2xNLS_human

    Plasmid

    #74491

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Tags

    t2a mCherry

    Expression

    Mammalian

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBLO1806_Cas9_2xNLS_human

    Plasmid

    #74493

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Tags

    t2a mCherry

    Expression

    Mammalian

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. LEXY TR (without KRAB domain) (pDN162)

    Plasmid

    #72661

    Purpose

    LexATF-T2A-NLS-LexA-mCherry-LEXY construct for light control of transcriptional repression

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Insert

    LexATF-T2A-NLS-LexA-mCherry-LEXY
    

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. LEXY TR (with KRAB domain) (pDN163)

    Plasmid

    #72662

    Purpose

    LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY construct for light control of transcriptional repression

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Insert

    LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY
    

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pREA12

    Plasmid

    #245770

    Purpose

    Allows for mCherry::RBD::T2A::rac-2::mGFP entry into Gateway Vectors

    Depositor

    Salvatore Cherra

    Article

    Lamb et al Development. 2025 Aug 1:dev.204678. doi:

    Insert

    mCherry::RBD::T2A::rac-2::mGFP
    

    Use

    Unspecified

    Tags

    mCherry and mGFP

    Available Since

    Sept. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pREA16

    Plasmid

    #245771

    Purpose

    Expresses mCherry::RBD::T2A::rac-2::mGFP in cholinergic motor neurons

    Depositor

    Salvatore Cherra

    Article

    Lamb et al Development. 2025 Aug 1:dev.204678. doi:

    Insert

    mCherry::RBD::T2A::rac-2::mGFP
    

    Tags

    mCherry and mGFP

    Expression

    Worm

    Promoter

    unc-17b

    Available Since

    Sept. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX458-3xHA-en31FnCas9ABEmax8.17d

    Plasmid

    #201956

    Purpose

    Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid

    #71829

    Purpose

    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    BACH2-sgRNA8 (BACH2 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    U6

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPL001-BBTK

    Plasmid

    #184751

    Purpose

    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    pEF1a-eGFP-T2A-BSD-bGHpA
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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