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pMAL728

Plasmid

#169152

Purpose

Encodes OptoQ-AMP6

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGC185

Plasmid

#19624

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChR2-GFP

Plasmid

#58881

Purpose

AAV expression of ChR2-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Oct. 30, 2014

Availability

Academic Institutions and Nonprofits only

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PKC delta dNPS

Plasmid

#16387

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC delta (Prkcd Mouse)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: aa 15…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
Id2 Ngfr

Plasmid

#80131

Purpose

overexpression of ID2

Depositor

Ellen Rothenberg

Article

Del Real et al Development. 2013 Mar;140(6):1207-19. do

Insert

Id2 (Id2 Mouse)

Use

Retroviral

Tags

IRES-Ngfr

Expression

Mammalian

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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CMVTO-mCherry-Kir2.1-HA-tevs-RXR

Plasmid

#179704

Purpose

TEVP-inducible mCherry-conjugated Kir2.1 RELEASE

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Kir2.1

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1

Plasmid

#187367

Purpose

Expresses human Plekhh1 labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRetroSuper Smad3

Plasmid

#15726

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad3 shRNA (SMAD3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5myc Rac1 N17

Plasmid

#15904

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Rac1 (RAC1 Human)

Tags

myc

Expression

Mammalian

Mutation

T17N

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRK5myc Cdc42 wt

Plasmid

#15905

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Cdc42 (placental isoform) (CDC42 Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pTetR193-Wasabi

Plasmid

#86105

Purpose

mWasabi reporter gene gated by temperature-sensitive TetR I193N

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TetRts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

I193N

Promoter

ptetA and ptetR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Golgi-targeted optodPLD

Plasmid

#140057

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pALM179

Plasmid

#82023

Purpose

an updated version of cLac143 (pACSA_PcptOO_C38T,A39T_YFP_PMB2_LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression

Depositor

Brian Pfleger

Article

Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

Insert

YFP

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDS244

Plasmid

#34982

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Ste11 (STE11 Budding Yeast)

Expression

Yeast

Promoter

ADH

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pLacI241-Wasabi

Plasmid

#86102

Purpose

mWasabi reporter gene gated by temperature-sensitive LacI A241T

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

LacIts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A241T

Promoter

T7A1O4/O3 and pLacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pPIDCB

Plasmid

#169899

Purpose

Empty dox inducible vector with piggyBac, insulators, and a DTS

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Avian expression

Expression

Mammalian

Promoter

Dox inducible

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-globin

Plasmid

#82582

Purpose

5' entry vector containing frog translation elongation factor 1 alpha (EF1a) enhancer fused to rabbit b-globin intron for semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

Frog EF1a enhancer fused to rabbit b-globin intron

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL630

Plasmid

#167836

Purpose

Encodes OptoLAC3 circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (AAV)

Expression

Bacterial

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP

Plasmid

#67299

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Tiam1-pMagFast2(3x)-iRFP (Tiam1 Mouse, Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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TC872

Plasmid

#164895

Purpose

TRE::nCre, inducible nCre expression when co-express with rtTA

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

nCre

Expression

Worm

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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