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pPSmOrange-C1

Plasmid

#31899

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PSmOrange

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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Ca-FLARE (protease)

Plasmid

#92214

Purpose

protease component of FLARE

Depositor

Alice Ting

Publication

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

CaM-V5-TEVp(Δ220-242)

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRK5 HA GST Rheb1

Plasmid

#14951

Depositor

David Sabatini

Publication

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

Rheb1 (RHEB Human)

Tags

HA-GST

Expression

Mammalian

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pZE21-GFPaav

Plasmid

#26643

Depositor

Michael Elowitz

Publication

Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

Insert

pZE21-GFPaav

Tags

GFPaav

Expression

Bacterial

Mutation

na

Available since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-ZNF598-TEV-3xFLAG

Plasmid

#105690

Purpose

Expresses C terminally 3xFLAG tagged ZNF598 protein

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Insert

ZNF598 (ZNF598 Human)

Tags

TEV-3xFLAG

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRK5 bovine GRK2

Plasmid

#14691

Depositor

Robert Lefkowitz

Publication

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GRK2 (GRK2 Bovine)

Expression

Mammalian

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
PL-SIN-EOS-C(3+)-EGFP

Plasmid

#21318

Purpose

Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP

Depositor

James Ellis

Publication

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

EOS-C(3+) promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Publication

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Promoter

UBC

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pVenus-VSVG

Plasmid

#11914

Depositor

Jennifer Lippincott-Schwartz

Publication

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VSVG

Expression

Mammalian

Mutation

F46L, F64L, M153T, V163A, S175G, T203Y, A206K rel…

Available since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMX-Brg1

Plasmid

#25855

Depositor

Hans Schöler

Publication

Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

Insert

Brahma related gene 1 (Smarca4 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCMV-HA-hIFITM1

Plasmid

#58399

Purpose

Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Publication

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM1 (IFITM1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEF1a-mMLH1dn

Plasmid

#174825

Purpose

Mammalian expression of mouse MLH1dn (codon optimized)

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mouse MLH1dn (codon optimized)

Expression

Mammalian

Mutation

deletion of residues 758-760

Promoter

EF1a

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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K44A dynamin 1 pEGFP

Plasmid

#34681

Depositor

Sandra Schmid

Publication

Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dynamin 1ba (DNM1L Human)

Tags

EGFP

Expression

Mammalian

Mutation

K44A

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMSCV mSin1.1 myc

Plasmid

#12576

Depositor

David Sabatini

Publication

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.1 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pET24b-APEX2

Plasmid

#111702

Purpose

For IPTG-inducible expression of APEX2 in E. coli

Depositor

Jessica Seeliger

Publication

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-EF1a-C-CreintG

Plasmid

#69571

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Has service

AAV1

Insert

kozak consensus - C-CreintG

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

Plasmid

#86233

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV-U6gRNA1-U6gRNA2-TnT-Cre

Plasmid

#87682

Purpose

AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

Depositor

William Pu

Publication

Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

Inserts

gRNA1
gRNA2
Cre

Use

AAV

Promoter

U6 and cTnT

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCSF107mT-GATEWAY-3'-FLAG

Plasmid

#67619

Purpose

A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Publication

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-terminal Flag

Expression

Mammalian

Promoter

CMV and SP6

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Publication

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Human, Synthetic)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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