We narrowed to 1,687 results for: plasmid for e coli

1. pXD70Tet(DadR-NFLAG)-N-3xFLAG-DadR

   Plasmid

   #191640

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR complementation

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   DadR
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CTL2 (M2225R)

   Plasmid

   #96952

   Purpose

   Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

   Depositor

   Douglas Rees

   Article

   Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

   Insert

   CTL2 (M222R) of PIEZO1
   

   Use

   Tags

   6x Histidine Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. FCM101

   Plasmid

   #194298

   Purpose

   E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6

   Depositor

   Shohei Koide

   Article

   Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:

   Insert

   FCM101
   

   Use

   Tags

   His tag, FLAG tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSW002-Pc-TorA(sp)-mNeonGreen

   Plasmid

   #205020

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   TorA-mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIA33

   Plasmid

   #120803

   Purpose

   E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

   Depositor

   David S. Weiss, Craig Ellermeier

   Article

   Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

   Inserts

   deactivated nuclease dCas9, codon-optimized for C. difficile
   single guide RNA targeting red fluorescent protein
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Base-pairing region targets red fluorescent prote…

   Promoter

   Pgdh and Pxyl

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30a-SARS-CoV2-NP

   Plasmid

   #165090

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pET26b_AT118-L-V5-His

   Plasmid

   #222848

   Purpose

   E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   V5-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30a-SARS-CoV2-NP-Nt(47-173)

   Plasmid

   #165091

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pSW002-Pc-TorT(sp)-mNeonGreen

   Plasmid

   #205021

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   TorT-mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET32a-Spike-S-4P

    Plasmid

    #165094

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pET30a-SARS-CoV2-ORF3b (S23Q)

    Plasmid

    #165088

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Stop codon to glutamine at 23rd amino acid

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET32a-Spike-403-505

    Plasmid

    #165095

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET30a-SARS-CoV2-ORF8

    Plasmid

    #165089

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pET26b_AT118-H-V5-His

    Plasmid

    #222850

    Purpose

    E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Use

    Tags

    V5-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET26b_AT118-A-V5-His

    Plasmid

    #222849

    Purpose

    E. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-A
    

    Use

    Tags

    V5-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-DEST42-ARL4D-V5-His6

    Plasmid

    #67426

    Purpose

    Expression of hArl4D with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid

    #186427

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    Rb7
    eGFP
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pExp-His-zBasic-RBD

    Plasmid

    #194999

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli

    Depositor

    Marko Hyvönen

    Article

    Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Tags

    His8-Zbasic

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX6P1-mCherry-Nanobody (LaM-2)

    Plasmid

    #162276

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    mCherry-Nanobody (LaM-2)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUDP004

    Plasmid

    #101165

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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