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FKBP-Stx3-mCitrine

Plasmid

#92428

Purpose

Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx3 (Stx3 Rat)

Tags

FKBP (FK506 binding protein 12); allows for heter…

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRM27

Plasmid

#163695

Purpose

ubb promoter driven expression of lck:mNeonGreen

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

lck:mNeonGreen (lck Zebrafish)

Use

Zebrafish expression

Tags

mNeonGreen

Promoter

ubb

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-Stx4-mCitrine

Plasmid

#92427

Purpose

Expression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx4 (Stx4 Rat)

Tags

FKBP (FK506 binding protein 12); allows for heter…

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO-sgRNA-puro

Plasmid

#104321

Purpose

3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

Depositor

Nathanael Gray

Article

Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1/TO

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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ARR3tk-eGFP/SV40-mCherry

Plasmid

#132360

Purpose

To measure transcriptional activity of Androgen Receptor

Depositor

Charles Sawyers

Article

Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

Insert

AR responsive elements (AR Human)

Use

Lentiviral

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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VAMP3(Δ71)-mCherry

Plasmid

#92425

Purpose

Expression of fusion incompetent VAMP3 (del71) C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

deleted leucine 71

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pWZ157

Plasmid

#163639

Purpose

pcn-1>PCN-1::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

pcn-1 (pcn-1 Nematode)

Use

CRISPR

Tags

GFP

Expression

Worm

Promoter

pcn-1

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pWZ186

Plasmid

#163641

Purpose

rps-27>DHB::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB:2xmKate2::3xHA

Use

CRISPR

Tags

2xmKate2

Expression

Worm

Promoter

rps-27

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pWZ194

Plasmid

#163640

Purpose

rps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Nematode, Synthetic)

Use

CRISPR

Tags

2xmKate2 and GFP

Expression

Worm

Promoter

rps-27

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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FRB_T2098L-VAMP3-mCherry

Plasmid

#92429

Purpose

Expression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

FKBP rapamycin binding (FRB) domain of mTOR with …

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAWK61

Plasmid

#163642

Purpose

rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Nematode, Human)

Use

CRISPR

Tags

2xmKate2 and GFP

Expression

Worm

Promoter

rps-27

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRM14

Plasmid

#163693

Purpose

hsp70 promoter inducible expression of DHB:mNeonGreen-P2A-H2B:mScarlet

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB:mNeonGreen-P2A-H2B:mScarlet (HELB, H2BC11 Human)

Use

Unspecified; Zebrafish expression

Tags

mNeonGreen and mScarlet

Promoter

HSP70

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRM15

Plasmid

#163694

Purpose

hsp70 promoter inducible expression of DHB:mScarlet-P2A-H2B:miRFP670

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB:mScarlet-P2A-H2B:miRFP670 (HELB, H2BC11 Human)

Use

Zebrafish expression

Tags

mScarlet and miRFP670

Promoter

HSP70

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_dSV40-H2B-GFP

Plasmid

#67928

Purpose

Expresses H2B-GFP in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

H2B-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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WNV VLPs pVRC8400

Plasmid

#63160

Purpose

West Nile Virus prM-E structural cassette (NY99 sequence) in pVRC8400

Depositor

Stephen Harrison

Article

Chao et al Elife. 2014 Dec 5;4. doi: 10.7554/eLife.

Insert

WNV prM-E

Expression

Mammalian

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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SL-25-0

Plasmid

#108673

Purpose

in vitro mRNA transcription

Depositor

Jon Lorsch

Article

Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

CAA repeat mRNA

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SL-150-0

Plasmid

#108677

Purpose

in vitro mRNA transcription

Depositor

Jon Lorsch

Article

Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

CAA repeat mRNA

Expression

Bacterial

Promoter

T7

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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C-Kras

Plasmid

#27807

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Cerulean Kras

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
CTA

Plasmid

#27804

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Cerulean Traf Amber

Expression

Mammalian

Available Since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
GH-t2a GFP

Plasmid

#194883

Purpose

an overexpression vector of the killifish growth hormone

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

growth hormone

Use

Lentiviral

Tags

t2a-GFP

Promoter

CMV

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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FSHB-GFP fused

Plasmid

#205596

Purpose

an overexpression vector of the killifish FSHB fused to GFP

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

FSHB

Tags

GFP

Promoter

CMV

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FSHB-t2a-dTOMATO

Plasmid

#205597

Purpose

an overexpression vector of the killifish FSHB

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

FSHB

Tags

dTOMATO

Promoter

CMV

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CAA 659

Plasmid

#108684

Purpose

in vitro mRNA transcription

Depositor

Jon Lorsch

Article

Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

CAA repeat mRNA

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
VTA

Plasmid

#27805

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Venus Traf Amber

Expression

Mammalian

Available Since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
APEX2-NLS

Plasmid

#124617

Purpose

proximity biotinylation

Depositor

Alice Ting

Article

Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

Insert

APEX2-NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-mCherry (527)

Plasmid

#70719

Purpose

Expression vector for mCherry purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

mCherry

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-UAS-CITRINE

Plasmid

#104839

Purpose

SPARK reporter gene

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

Citrine

Use

AAV

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CTV

Plasmid

#27803

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Cerulean Traf Venus

Use

Fret standard

Expression

Mammalian

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pET21a(+)-Histag-Cerulean (526)

Plasmid

#70716

Purpose

Expression vector for Cerulean purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Cerulean

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
V-Kras

Plasmid

#27806

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Venus kras

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pET21a(+)-Histag-Citrine (525)

Plasmid

#70718

Purpose

Expression vector for Citrine purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DOX: FSHB-t2a-GFP

Plasmid

#205595

Purpose

an overexpression vector of the killifish FSHB under doxycycline control

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

FSHB

Tags

GFP

Promoter

Ef1a

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-UAS-Luciferase

Plasmid

#104840

Purpose

SPARK reporter gene

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

Luciferase

Use

AAV

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX208-beta-arrestin2-HA-TEV219

Plasmid

#104846

Purpose

b2AR-SPARK protease component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

beta-arrestin2-HA-TEV219

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
3n1 (572)

Plasmid

#70727

Purpose

BetI/PhlF/SrpR BCD7 3-node v41-1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Inserts

srpR-ssrA
betI-ssrA
phlF-ssrA

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-mCherry-P2A-strepII-PPPCB

Plasmid

#67923

Purpose

Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-beta-arrestin2-HA-TEV219

Plasmid

#104845

Purpose

b2AR-SPARK protease component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

beta-arrestin2-HA-TEV219

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NFfshb-t2a-GFP

Plasmid

#194356

Purpose

over expression vector for killifish fshb tagged with GFP

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

follicle stimulating hormone beta subunit

Use

Lentiviral

Tags

t2a-GFP

Expression

Mammalian

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FJZ 663

Plasmid

#108689

Purpose

in vitro mRNA transcription

Depositor

Jon Lorsch

Article

Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

RPL41A (RPL41A Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FJZ 659

Plasmid

#108690

Purpose

in vitro mRNA transcription

Depositor

Jon Lorsch

Article

Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

RPL41A (RPL41A Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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