We narrowed to 4,243 results for: Pet2;

1. pET28-his6-HA3-CLIP

   Plasmid

   #188942

   Purpose

   T7 expression vector for making his6- and HA3-tagged CLIP protein

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   his6-HA3-CLIP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22-6His-SUMO-Nsp10

   Plasmid

   #154960

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   July 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET21-GFP-AURKA-mCherry

   Plasmid

   #99876

   Purpose

   Expression of GFP-AURKA-mCherry for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-hDcp2 E147Q, E148Q

   Plasmid

   #72215

   Purpose

   bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E147Q, E148Q

   Promoter

   T7

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET24a-VHH-tev-APEX2

   Plasmid

   #117751

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

   Insert

   anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-Hook3 aa 1-160-GCN4

   Plasmid

   #74608

   Purpose

   Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-160 (HOOK3 Human)
   

   Use

   Tags

   6x His, GCN4, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pet22b-mEGFP-YAP-N-IDR

   Plasmid

   #166440

   Purpose

   Bacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion protein

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, YAP-N-IDR(1-164) (YAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET23b-Casp7 C186A-His

   Plasmid

   #11826

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 7 catalytic mutant (CASP7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C186A

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

9. WT N-His Ana GvpC in pET28a

   Plasmid

   #153294

   Purpose

   Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Gas vesicle protein C (N-terminal his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added a hexahistidine tag after the methionine at…

   Promoter

   T7

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-sfGFP[D134TAG+N150TAA]

    Plasmid

    #164582

    Purpose

    superfolder GFP with D134TAG and N150TAA mutations

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Insert

    sfGFP-134TAG+150TAA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D134TAG + N150TAA Mutations

    Promoter

    T7

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pF714-pET28a-Hs-FL-METTL20-NHis wt

    Plasmid

    #85115

    Purpose

    expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pET28a(+) 6xHis-TEV-IFIT5

    Plasmid

    #53560

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Use

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET28a-Hisx6-Sirt6-H133Y

    Plasmid

    #20279

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Histidine 133 to Tyrosine

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pET28-Strep-TEV-Nsp14

    Plasmid

    #154955

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Strep-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET28-His6-Ubc9-C93A

    Plasmid

    #133910

    Purpose

    Bacterial expression of His6-Ubc9-C93A

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Use

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    Cysteine 93 to Alanine

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-Gluc-EL222-his

    Plasmid

    #172097

    Purpose

    Expresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteria

    Depositor

    Avi Schroeder

    Article

    Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

    Insert

    Gaussia Luciferase - EL222
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gluc mutations - M43L, M110L

    Promoter

    T7

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b(+)_S. cerevisiae Hbs1(1-611)

    Plasmid

    #39336

    Depositor

    SeWon Suh

    Insert

    Elongation factor 1 alpha-like protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pET21a(+) LdtMt2 (131-408)

    Plasmid

    #59337

    Purpose

    Expresses LdtMt2 for L,D-transpeptidation

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    LdtMt2 (131-408)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pET28-sfGFP-SpyTag003 Loop A

    Plasmid

    #171776

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop A
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues V…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28-MRPP3-myc-His

    Plasmid

    #67864

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP3 (KIAA0391 Human)
    

    Use

    Tags

    His and myc

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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