We narrowed to 4,205 results for: SUP

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pCW57.1_V5_BirA*_FLAG_Nt

Plasmid

#194070

Purpose

3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Type

Empty backbone

Use

Lentiviral

Tags

V5-BirA(R118G)-FLAG

Expression

Mammalian

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-intron

Plasmid

#177804

Purpose

An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

intron

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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PB-Ostir-neo

Plasmid

#161973

Purpose

Expresses Ostir and G418 resistance gene in mammalian cells

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Ostir-3xHA-p2a-neoR

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSECC-sgTom

Plasmid

#138661

Purpose

Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgTomato

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307 GFP-Kif26b-C

Plasmid

#118483

Purpose

lentiviral expression of GFP-Kif26b-C in mammalian cells

Depositor

Henry Ho

Article

Karuna et al Genes (Basel). 2018 Apr 5;9(4). pii: gen

Insert

mouse Kif26b-C (Kif26b Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Encodes GFP fused to amino acid 1735 to 2112 of m…

Promoter

EF1A

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDN-G1Gh

Plasmid

#44507

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
yEGFP

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pBSSK p85 alpha (delta SH3, iSH2)

Plasmid

#13433

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta SH3, iSH2) (PIK3R1 Human)

Tags

Flag

Expression

Bacterial

Mutation

Flag tags replacing both the SH3 and iSH2 domains.

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
pAID1.2-EF1a-CmCherry-mAID

Plasmid

#140604

Purpose

Expression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGRE GFP

Plasmid

#1196

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

GFP w/ stop codon

Expression

Yeast

Mutation

GFP w/ stop codon

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pTA-Mob 2.0 Tp

Plasmid

#188619

Purpose

Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Conjugation helper plasmid

Expression

Bacterial and Yeast

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluN2B TKIT donor

Plasmid

#169431

Purpose

Donor DNA for TKIT knockin of SEP to NR2B in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFLuc190UGA

Plasmid

#41046

Depositor

James Inglese

Article

Auld et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Firefly luciferase 190UGA

Expression

Mammalian

Mutation

in-frame UGA stop codon was created at codon 190

Promoter

CMV

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pSK ZO1

Plasmid

#30316

Depositor

James Anderson, Alan Fanning

Article

Willott et al Proc Natl Acad Sci U S A. 1993 Aug 15. 9

Insert

Zonula Occludens-1 (TJP1 Human)

Use

Subcloning

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4

Plasmid

#193345

Purpose

tetO-OS*K (Dox-inducible reprogramming lentiviral OSK vector expressing mouse Oct4 + super-Sox + Klf4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Sox2-17-t2a-Klf4 (Klf4, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-AncMEcoGII291

Plasmid

#205024

Purpose

Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

Depositor

Paola Laurino

Article

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

Ancestral M.EcoGII 291

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pM201

Plasmid

#173179

Purpose

dCas9-24xGCN4

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

dCas9-24xGCN4

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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miR-vector-RFP pCDNA

Plasmid

#97143

Purpose

for expression of miRNA and TagRFP

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Tags

TagRFP

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad2-3'UTR

Plasmid

#61775

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pLV_iRFP_P2AT2A_mScarlet-I_Myl9

Plasmid

#172474

Purpose

Mammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.

Depositor

Sean R Collins

Article

Hadjitheodorou et al Nat Commun. 2021 Nov 16;12(1):6619. doi:

Inserts

iRFP670
mScarlet-I-Myl9 (MYL9 Human)

Use

Lentiviral

Tags

mScarlet-I

Expression

Mammalian

Promoter

EF1alpha (with UCOE) and none (same transcript as…

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Gal4 PGC1 alpha L2/3A

Plasmid

#8898

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

PGC-1a (Ppargc1a Mouse)

Tags

Gal4 DBD

Expression

Mammalian

Mutation

LXXLL at aa140 is changed to AXXLL; LLXXL at aa 2…

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only