164,728 results

1. Lenti-multi-CRISPR

   Plasmid

   #85402

   Purpose

   Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL-MS2-12X

   Plasmid

   #27119

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2 binding sites (12)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSH-EFIRES-B-Seipin-miniIAA7-mEGFP

   Plasmid

   #129719

   Purpose

   AAVS1 safe harbor targeting vector expressing miniIAA7-mEGFP tagged Seipin

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   Seipin (BSCL2 Human)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   miniIAA7-mEGFP

   Expression

   Mammalian

   Mutation

   silent mutations

   Promoter

   EF1a

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-iRFP670-CaaX

   Plasmid

   #170464

   Purpose

   Expresses iRFP670 fluorophore targeted to the plasma membrane using a prenylation motif

   Depositor

   John James

   Article

   Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

   Insert

   iRFP670-CaaX
   

   Use

   Lentiviral

   Tags

   CaaX

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-CaMPARI

   Plasmid

   #60421

   Purpose

   Expresses CaMPARI in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Loren Looger, Eric Schreiter

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI
   

   Use

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. AbVec2.0-mIghg1

   Plasmid

   #127158

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype

   Depositor

   Hedda Wardemann

   Insert

   Ighg1 (Ighg1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-mito-GEM-GECO1

   Plasmid

   #32461

   Purpose

   Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEM-GECO
   

   Use

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

   Promoter

   CMV

   Available since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV9)

   Viral Prep

   #162380-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   CAG

   Tags

   None

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pCH1/RAD51o

   Plasmid

   #102562

   Purpose

   Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

   Depositor

   Maria Spies

   Article

   Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

   Inserts

   Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
   E. coli groE operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli Expression

   Promoter

   T7

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T1_ATG4B_BamHI_NotI

    Plasmid

    #190862

    Purpose

    Plasmid for the expression and purification of of ATG4B. Internal reference: SMC1392

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    ATG4B (ATG4B Human)
    

    Use

    Tags

    GST, Thrombin Cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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