We narrowed to 15,920 results for: T7

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pcDNA-HIRA-polyA

Plasmid

#59781

Purpose

Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-emerin-EGFP-polyA

Plasmid

#59747

Purpose

Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

emerin-EGFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-EGFP-ELYS-polyA

Plasmid

#59746

Purpose

Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

EGFP-ELYS

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pDB023f1

Plasmid

#99218

Purpose

Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD4] T7RNAP(S12*,S203*)

Expression

Bacterial

Mutation

amber codons at positions 12 and 203 of T7 RNA po…

Promoter

Ppsp

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pet28a-ClbB-NRPS-NHis

Plasmid

#49217

Purpose

NRPS module of ClbB containing CAT domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbB-NRPS

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa4-1080 of reference sequence NP_754362…

Promoter

T7

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-RCC1-polyA

Plasmid

#59762

Purpose

Express Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

RCC1

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pET23a-Mm/HsUBA52

Plasmid

#69560

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBA52 (UBA52 Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pDB023f

Plasmid

#99217

Purpose

Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] T7RNAP(S12*,S203*)

Mutation

amber codons at positions 12 and 203 of T7 RNA po…

Promoter

Ppsp

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pTHSSd_27

Plasmid

#59958

Purpose

PTac null fragment (sigma CGG Y639A) expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

null fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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bbCas9pluspAAA

Plasmid

#82581

Purpose

The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA

Depositor

Ivo Huijbers

Article

Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

Insert

Cas9

Expression

Bacterial

Promoter

T7

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDB023k

Plasmid

#99219

Purpose

Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] T7RNAP(S12*,S203*,S527*)

Mutation

amber codons at positions 12, 203, and 527 of T7 …

Promoter

Ppsp

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pTHSSd_38

Plasmid

#59961

Purpose

High core fragment expression (high resource allocator)

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

core fragment

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_1

Plasmid

#59947

Purpose

Splitposon in KanR ColE1 backbone

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

Splitposon w/ KanR, LacI & PTac

Expression

Bacterial

Mutation

N/A

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-ClbN-NHis

Plasmid

#51497

Purpose

NRPS module containing CATE domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbN

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa30-1094 of NP_754347.1

Promoter

T7

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBC

Plasmid

#69555

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBC (Ubc Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pARiBo1

Plasmid

#98892

Purpose

Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

ARiBo1

Use

In vitro transcription vector

Promoter

T7 promoter

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBB

Plasmid

#69556

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pTHSSd_39

Plasmid

#59962

Purpose

Low core fragment expression (low resource allocator)

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

core fragment

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_40

Plasmid

#59963

Purpose

High core fragment expression without SynZIP

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

core fragment lacking SynZIP

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50013

Plasmid

#50312

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal T7 tag (bacteriophage T7 gene 10)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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