We narrowed to 4,154 results for: pCMV

1. pCMV5 HA-TBRII (delta Cyt)

   Plasmid

   #14051

   Depositor

   Joan Massague

   Article

   Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

   Insert

   TGF beta rec II (delta cyt) (TGFBR2 Human)
   

   Use

   Tags

   HA (PYDVPDYA)

   Expression

   Mammalian

   Mutation

   Dominant negative mutant. A stop codon was introd…

   Promoter

   Available since

   May 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hA3A-BE3-D131Y

   Plasmid

   #113424

   Purpose

   Expresses hA3A-BE3-D131Y in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-BE3-D131Y (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_D131Y

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Myc CDC4 R465C

   Plasmid

   #16653

   Depositor

   Bert Vogelstein

   Article

   Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

   Insert

   CDC4 R465C (FBXW7 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R465C

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pCMVlac-dirTopo-AU1-aGFP

   Plasmid

   #61971

   Purpose

   A directional cloning vector of PCR-amplified cDNAs for mammalian expression

   Depositor

   Hiroshi Udo

   Inserts

   lacZ alpha
   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   It contains silent mutations

   Promoter

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVlac-dirTopo-P2A-aGFP

   Plasmid

   #61972

   Purpose

   A directional cloning vector of PCR-amplified cDNAs for mammalian expression

   Depositor

   Hiroshi Udo

   Inserts

   lacZ alpha
   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   It contains silent mutations

   Promoter

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

   Plasmid

   #163561

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-HF-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-RosaL6 ELD mutations

   Plasmid

   #37198

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

   Insert

   FokI (fused to Rosa L6 ZFN)
   

   Use

   Zfn expression

   Tags

   HA, ROSA26 eZFNs L6, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Q486E, I499L and N496D

   Promoter

   CMV

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-eGFP-NMHC-IIAΔtailpiece

   Plasmid

   #35689

   Depositor

   Tom Egelhoff

   Article

   Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

   Insert

   MHC IIA (MYH9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Added stop codon after codon 1928, eliminating th…

   Promoter

   CMV

   Available since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-FLAG-LOV*-MVP

   Plasmid

   #202206

   Purpose

   Expresses the photoproximity labeling catalyst LOV* fused to MVP in mammalian cells

   Depositor

   Thomas Muir

   Article

   Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

   Insert

   Major vault protein (MVP Human)
   

   Use

   Tags

   FLAG and LOV*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-FKBP-mCherry-MCS

    Plasmid

    #178863

    Purpose

    Mammalian expression of a protein of interest fused to the C-terminus of FKBP-mCherry

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    FKBP-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCMV-intron myc Rab6WT

    Plasmid

    #46781

    Purpose

    expression of WT Rab6a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    Rab6 (RAB6A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-VSV-G(P127D)-Myc

    Plasmid

    #80055

    Purpose

    Expression of a Myc tagged VSV-G protein

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VSV-G-Myc(P127D)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P127D

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-AsCas12f1-v5.1+T1-empty

    Plasmid

    #204977

    Purpose

    AsCas12f1-v5.1+T1-based Human cell genome editing

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nature Catalysis (2023) 6, 695–709

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    N70Q/K103R/A104R/S118A/D364R

    Promoter

    PCMV,U6

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_UdgX-APOBEC1-UdgX-nCas9

    Plasmid

    #163555

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV4a-SIRT6-H133Y-FLAG

    Plasmid

    #102324

    Purpose

    Human SIRT6 H133Y with FLAG tag for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed histidine 133 to tyrosine

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV 3xFLAG-LC3B G120

    Plasmid

    #123094

    Purpose

    Expresses 3xFLAG-LC3B G120 in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV5B-TFGbeta receptor II K277R

    Plasmid

    #11762

    Depositor

    Joan Massague, Jeff Wrana

    Article

    Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

    Insert

    TGFbeta receptor II (TGFBR2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    K277R: kinase dead

    Promoter

    CMV

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCMV-eGFP-NMHC-IIAdeltaIQ2

    Plasmid

    #35690

    Depositor

    Tom Egelhoff

    Article

    Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

    Insert

    MHC IIA (MYH9 Human)
    

    Use

    Tags

    EGFP and GFPGenotRW

    Expression

    Mammalian

    Mutation

    The coding segment for aa 804-834 (corresponding …

    Promoter

    CMV

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCMV 3xFLAG-LC3B G120A

    Plasmid

    #123093

    Purpose

    Expresses 3xFLAG-LC3B G120A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_UdgX-APOBEC1-UdgX-HF-nCas9

    Plasmid

    #163559

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-HF-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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