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We narrowed to 10,124 results for: Met

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Showing: 1641 - 1660 of 10124 results

  5. pDule2-Tet2.0

    Plasmid
    #85497
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Publication
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    85497-plasmid-map-sequence-id-169854

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  11. pALS2-sfGFP WT

    Plasmid
    #197575
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication
    Depositor
    Ryan Mehl
    Publication
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6)
    Tags
    His6
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197575-plasmid-map-sequence-id-396574

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  12. pAcBac1.tR4-AcdRS82

    Plasmid
    #174101
    Purpose
    Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.
    Depositor
    Ryan Mehl
    Publication
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    M. barkeri tRNA synthetase for acridone82
    Expression
    Mammalian
    Mutation
    N311S, C313G, V366A, W382T
    Promoter
    CMV
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174101-plasmid-map-sequence-id-345718

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  14. pAS_4xMma PylT sfGPF 150TAG

    Plasmid
    #154766
    Purpose
    plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Publication
    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021
    Insert
    sfGFP
    Expression
    Mammalian
    Mutation
    150TAG in GFP reporter, U25C in PylT
    Promoter
    EF1
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    154766-plasmid-map-sequence-id-309629

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  15. pBV65

    Plasmid
    #108363
    Purpose
    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.
    Depositor
    Scott Moye-Rowley
    Publication
    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0
    Insert
    loxP-natMX6-CgMET3prom-cre-loxP
    Use
    Gene disruption marker for candida glabrata
    Promoter
    C. glabrata MET3
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108363-plasmid-map-sequence-id-209915

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  16. pMega-MaFRSA

    Plasmid
    #200226
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..
    Depositor
    Alanna Schepartz
    Publication
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutated Asparagine 166 to Alanine and Valine 168 …
    Promoter
    proK-lacO and tacI
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200226-plasmid-map-sequence-id-393639

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Showing: 1641 - 1660 of 10124 results