We narrowed to 5,560 results for: pcas

1. pCAG-IRES2-p75NTR-GFP

   Plasmid

   #182947

   Purpose

   To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal

   Depositor

   Pantelis Tsoulfas

   Insert

   p75NTR-mGFP
   

   Use

   Tags

   HA tag and mGFP

   Expression

   Mammalian

   Mutation

   The leader sequence is from preprotrypin

   Promoter

   CAG

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

   Plasmid

   #98975

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 4 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Bmpr2ΔKinase gRNA resistant

   Plasmid

   #163411

   Purpose

   Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The kinase domain (202-500 aa) is deleted. gRNA-t…

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-GBP6-10gly-VPminx4

   Plasmid

   #50796

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP6-VPminx4 activation domain fusion protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-SARS2-S-D614 (NC-flag)

   Plasmid

   #156418

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-loxpSTOPloxp-lyn-NeonGreen

   Plasmid

   #175257

   Purpose

   membrane target protein labelling

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   Lyn membrane targeting motif
   

   Use

   Cre/Lox

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   pCAG

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAX APPs-751-alpha

   Plasmid

   #30149

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein - soluble (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   splice variant 751; amino acids 1–612

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCaUra3-CUbo-AID*-GFP

   Plasmid

   #212775

   Purpose

   C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-HA-rat Nrxn1beta AS4(-)

   Plasmid

   #58267

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn1beta AS4(-) (Nrxn1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   see comments

   Promoter

   CAG

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-nDsRedFL-miR9/9*/124

    Plasmid

    #177807

    Purpose

    The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-9/9*/124
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS/XBB.1.5-RBD

    Plasmid

    #213038

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/XBB.1.5-RBD (S )
    

    Use

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Gag-FKBP-Myc

    Plasmid

    #177909

    Purpose

    HIV Gag-FKBP-Myc tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    HIV Gag (gag )
    

    Use

    Tags

    FKBP12 and Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid

    #191388

    Purpose

    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_hSLBP(56_108)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-SRGAP1FBAR(516a.a.)-EGFP

    Plasmid

    #168505

    Purpose

    Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP1-FBAR (SRGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-516

    Promoter

    CAG

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-pac-T2A-dre

    Plasmid

    #124833

    Purpose

    Puromycin resistance gene and dre recombinase

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-dre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-opCas12a-Ultra-6xNLS

    Plasmid

    #209024

    Purpose

    Lentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    opCas12a-Ultra-6xNLS-Flag-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and MYC

    Expression

    Mammalian

    Mutation

    E174R/M537R/S542R/F870L

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCASP-SptP120-K55-HilA

    Plasmid

    #153329

    Purpose

    Triggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-K55 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-CIBN-EGFP-KRasCT

    Plasmid

    #178527

    Purpose

    Expresses CIBN-EGFP-KRasCT in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CIBN-EGFP-KRasCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-HA-rat Nrxn2beta AS4(-)

    Plasmid

    #58268

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn2beta AS4(-) (Nrxn2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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