We narrowed to 4,168 results for: 1-Oct

1. FUW TetO HA-AMPKa2 AS

   Plasmid

   #69827

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV promoter with tetracycline operator

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

   Plasmid

   #227800

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA 2 (Vegfa Synthetic)
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3

   Plasmid

   #227801

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA 3 (Vegfa Synthetic)
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRaf-IRES-pErk reporter

   Plasmid

   #226170

   Purpose

   Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)
   

   Use

   Tags

   3xFlag-tdMCP and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-KMS

   Plasmid

   #188038

   Purpose

   Polycistronic human KLF4, MYC and SOX2 were cloned into the pMXs vector

   Depositor

   Young Tang

   Article

   Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

   Insert

   KLF4, MYC, and SOX2 separated by 2A sequences (KLF4, MYC, SOX2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC

   Plasmid

   #229545

   Purpose

   Lentiviral construct to express rtTA and SKM proteins

   Depositor

   Domitilla Del Vecchio

   Article

   Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

   Insert

   rTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMasterK

   Plasmid

   #165081

   Purpose

   Episomal expression of reprogramming factors.

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

   Inserts

   OCT4 (POU5F1 Human)
   SOX2 (SOX2 Human)
   KLF4 (KLF4 Human)
   MYC (MYC Human)
   LRH1 (NR5A2 Human)
   NANOG (NANOG Human)
   lin28 (LIN28A Human)
   EGFP
   HSV TK
   KDM4D (KDM4D Callithrix jacchus)
   GLIS1 (GLIS1 Human)
   

   Use

   Episomal

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCXLE-Sox2AV-T2A-Klf4-E2A-Myc

   Plasmid

   #210017

   Purpose

   episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset.

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Sox2A61V is a point mutant of Sox2 that enhances …

   Promoter

   CAG

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pnCS_SEPT7_SEPT9_i1-TEV-Strep

   Plasmid

   #174500

   Purpose

   bacterial co-expression of human SEPT7 and of human SEPT9_i1

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 Human)
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnEA-vH_His-TEV-DSep1-msfGFP

    Plasmid

    #174494

    Purpose

    bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Insert

    Sep1 (Sep1 Fly)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUW-SO

    Plasmid

    #20329

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Sox2-F2A-Oct4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FUW-KO

    Plasmid

    #20331

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Klf4-F2A-Oct4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TMEM16A-I546K-peGFP-N1

    Plasmid

    #228582

    Purpose

    Expresses mouse TMEM16A-I546K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I546K

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TMEM16A-I547K-peGFP-N1

    Plasmid

    #228583

    Purpose

    Expresses mouse TMEM16A-I547K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I547K

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TMEM16A-E551K-peGFP-N1

    Plasmid

    #228584

    Purpose

    Expresses mouse TMEM16A-E551K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E551K

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCM-CLYBL-NGN2-BRN3A

    Plasmid

    #165597

    Purpose

    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.

    Depositor

    Carsten Bönnemann

    Article

    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…

    Available since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. NTHL1-mCherry

    Plasmid

    #215145

    Purpose

    Expresses NTHL1-mCherry in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    NTHL1 (NTHL1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES2-smGFP-Kv2.1

    Plasmid

    #131709

    Purpose

    Expresses Kv2.1 in mammalian cells

    Depositor

    Jennifer Kearney

    Article

    Kang et al Ann Neurol. 2019 Dec;86(6):899-912. doi:

    Insert

    KCNB1 (KCNB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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