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pME_Cp_0_2-3a_004_atpA_Promoter+5'UTR_Cr

Plasmid

#235876

Purpose

atpA promoter + 5'UTR Chlamydomonas reinhardtii 2-3a

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

atpA promoter + 5'UTR

Use

Synthetic Biology

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_2-3_005_Promoter+5'UTR_Placeholder

Plasmid

#235870

Purpose

Encodes a sfGFP cassette in the promoter+5'UTR position used for placeholder cloning

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Promoter + 5'UTR placeholder

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Flag- Src H492A

Plasmid

#246425

Purpose

Expresses Src mutant H492A (aka H495A) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Histidine 492 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src Y527A

Plasmid

#246429

Purpose

Expresses Src mutant Y527A (aka Y530A) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Tyrosine 530 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A

Plasmid

#244492

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src H201A

Plasmid

#246424

Purpose

Expresses Src mutant H201A (aka H204A) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed His 204 to Ala

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 SHP2 E252A

Plasmid

#244491

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Glutamate 252 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src E524A

Plasmid

#246426

Purpose

Expresses Src mutant E524A (aka E527A) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Glu 527 to Ala

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src C185A

Plasmid

#246423

Purpose

Expresses Src mutant C185A or C188A in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Cys 188 to Ala

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244076

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244100

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.H348H.HaloTag

Plasmid

#244086

Purpose

Cytosolic expression of green glucose sensor (high affinity) with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Use

AAV

Tags

HaloTag

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244087

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244080

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244083

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(mem).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244102

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pME-DRE-nls (JDW 31)

Plasmid

#242561

Purpose

Gateway middle entry clone containing Dre recombinase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

codon optimized DRE-nls

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-LiCre (JDW 1119)

Plasmid

#242560

Purpose

Gateway middle entry clone containing a light inducible Cre Recombinase (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre (AsLOV2-CreE340AD341A)

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-SV40-pA (JDW 1417)

Plasmid

#242570

Purpose

Gateway 3' entry clone containing mCherry followed by an SV40 polyA.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCherry stop SV40 pA

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-miR-MCS (JDW 1231)

Plasmid

#242562

Purpose

Gateway middle entry clone containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with artifial intron and MCS

Use

Gateway subcloning

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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