We narrowed to 7,670 results for: URE

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pHR Grb2-TagBFP

Plasmid

#188631

Purpose

Human Grb2 C-terminally labeled with TagBFP

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

GRB2 (GRB2 Synthetic, Human)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX neo ACTN1-GFP

Plasmid

#138293

Purpose

Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

ACTN1 (ACTN1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS-N-HA

Plasmid

#130913

Purpose

Expresses human cGAS N terminus(aa1-159)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS N terminus

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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CYFIP2.mCherry

Plasmid

#122052

Purpose

Expresses human CYFIP2 in mammalian cells.

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP2 (CYFIP2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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STT3A pEGFP-N2 (1051)

Plasmid

#62025

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

STT3A (STT3A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pMOD4 GFP

Plasmid

#19181

Depositor

Al Fisher

Article

Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

Insert

Green fluorescent protein

Tags

FLAG and daf-12 N-term 328 amino acids

Expression

Worm

Mutation

S65C, contains synthetic introns for C. elegans

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

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paavCAG-Jx

Plasmid

#51905

Purpose

Cre-dependent On/Off aav vector

Depositor

Jinhyun Kim

Article

Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

Type

Empty backbone

Use

AAV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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ttAtCas12a+int

Plasmid

#182384

Purpose

Gives very high editing efficiency in barley. Also works in Brassica oleracea

Depositor

Wendy Harwood

Article

Lawrenson et al bioRxiv 2022.04.28.489853

Insert

LbCas12a coding sequence with D156R and Arabidopsis introns

Use

CRISPR

Mutation

D156R + 8 Arabidopsis introns added

Promoter

None

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:

Plasmid

#179585

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NLS-NG-T2A

Promoter

CAGp

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNRDc-V5

Plasmid

#51297

Purpose

expression of mouse NRDc in mammalian cells

Depositor

Eiichiro Nishi

Article

Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

Insert

NRDc (Nrd1 Mouse)

Tags

HIS and V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
Integrin alpha5(1-623)-AHCys

Plasmid

#27301

Depositor

Timothy Springer

Article

Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

Insert

integrin alpha 5 (ITGA5 Human)

Expression

Mammalian

Mutation

alpha 5(1-623)+GG+TEV+GGEN+AHCys

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only
pSaPIPLC His

Plasmid

#173810

Purpose

Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

Depositor

Anne Gershenson, Mary F Roberts

Article

Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

Insert

Staphylococcus aureus plc

Tags

6XHis

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA7woH

Plasmid

#65020

Purpose

Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, E105K, E108A, D16…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast

Plasmid

#205540

Purpose

Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

TRE3GS

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pUltraHot-mCherry-DHX36-iso1-WT

Plasmid

#206964

Purpose

expressing the protein DHX36 fused to mCherry in human cells

Depositor

Eran Hornstein

Article

Danino et al Nucleic Acids Res. 2023 Jul 28:gkad613.

Insert

DEAH-Box Helicase 36 (DHX36 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONOR223 RpH-LAMP1-3xFLAG

Plasmid

#163019

Purpose

gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

RpH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Gateway system

Tags

3XFLAG

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry-FKBP-GEF(TIAM1)

Plasmid

#85156

Purpose

acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Fusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)

Tags

mCherry-FKBP

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

Plasmid

#60492

Purpose

Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm

Depositor

Dorus Gadella

Article

Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

Inserts

3xnls-SGFP2(T65G)
SGFP2-3xNES

Tags

SGFP2 and SGFP2(T65G)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA HisB TO rFez1

Plasmid

#17385

Depositor

Stephen Goff

Article

Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

Insert

FEZ1 (Fez1 Rat)

Tags

his and myc

Expression

Mammalian

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Plasmid

#182063

Purpose

Co-expression of Arch-based voltage indicator (QuasAr6a) and membrane-localized calcium reporter (jRGECO1a-CAAX)

Depositor

Adam Cohen

Article

Landau et al eLife. 2022 Mar 23;11. pii: 76993. doi:

Insert

QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Use

Lentiviral

Promoter

CAG

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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