We narrowed to 7,766 results for: loxP

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pUG-amdSYM

Plasmid

#254144

Purpose

Carries the Aspergillus nidulans expression cassette flanked by LoxP sites; suitable template for deletion cassettes containing the new marker module amdSYM

Depositor

Jean-Marc Daran

Publication

Solis-Escalante et al FEMS Yeast Res. 2013 Feb;13(1):126-39. d

Insert

acetamidase

Expression

Yeast

Promoter

TEF promoter from Ashbya gossypii

Available since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40

Plasmid

#100831

Purpose

AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Publication

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLP-PIGA3 (pLP305)

Plasmid

#196992

Purpose

Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.

Depositor

Matthew Maurano

Publication

Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Expression

Mammalian

Promoter

EF1a

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-jYCaMP1s

Plasmid

#135420

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

Depositor

Kaspar Podgorski

Publication

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Has service

AAV1

Insert

jYCaMP1s

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDD315 (mTurquoise2^SEC^2xHA)

Plasmid

#73343

Purpose

mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Publication

Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

Insert

mTurquoise2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

2xHA and C. elegans codon-optimized mTurquoise2

Expression

Worm

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209655

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Has service

AAV5

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Mutation

S29W.A95K

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.iGluSnFr.WPRE.SV40

Plasmid

#98932

Purpose

Cre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSICO-Flpo

Plasmid

#24969

Depositor

Tyler Jacks

Publication

Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 10

Insert

Flp recombinase

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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TRIPZ-bioGEF-UBnc-PURO

Plasmid

#208044

Purpose

Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Publication

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

UBnc (UBC Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Mutation

L73P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC 3GLA

Plasmid

#71762

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant

Depositor

Matthias Soller

Publication

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Expression

Insect

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDD317 (Pmyo-2::GFP + SEC)

Plasmid

#73344

Purpose

SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Publication

Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

Insert

Pmyo-2::GFP + SEC

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Promoter

myo-2

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIPZ

Plasmid

#206981

Purpose

empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

Depositor

Ming Chen

Publication

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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LGmCreER (self deleting)

Plasmid

#33340

Depositor

Scott Lowe

Publication

Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

Insert

CreERT2

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

PGK

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pORANGE LOX Cloning template vector

Plasmid

#139651

Purpose

Cloning template for ORANGE-CAKE conditional knock-in constructs

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTRIPZ shSTING-Hsa

Plasmid

#128158

Purpose

Doxycyclin inducible shRNA knockdown of human STING gene

Depositor

Sandra Demaria

Publication

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shSTING Hsa (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-CA-Src-GFP

Plasmid

#83469

Purpose

Lentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cells

Depositor

Barry Gumbiner

Publication

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Src (SRC Chicken)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Y527F (constitutively active)

Promoter

doxycycline-inducible promoter

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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TRIPZ-bioGEF-UBwt-PURO

Plasmid

#208045

Purpose

Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Publication

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

Ub (UBC Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Promoter

tetO/UBC

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTRIPZ shcGAS-Hsa

Plasmid

#128175

Purpose

Doxycyclin inducible shRNA knockdown of human cGAS gene

Depositor

Sandra Demaria

Publication

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shcGAS Mmu (CGAS Human)

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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WT PBRM1- TRIPZ-neo

Plasmid

#107406

Purpose

Mammalian expression, inducible PBRM1

Depositor

William Kaelin

Publication

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

PBRM1 (PBRM1 Human)

Use

Lentiviral

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTRIPZ-EGFP:Ajuba

Plasmid

#108229

Purpose

Lentiviral vector for Tet-inducible Ajuba expression

Depositor

Kenneth Irvine

Publication

Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

Insert

AJUBA (AJUBA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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