We narrowed to 13,845 results for: cas9

1. Lenti-sgNT/Cre

   Plasmid

   #66895

   Purpose

   Expresses a non-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

   Insert

   sgNT
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiGuide-Crimson

   Plasmid

   #70683

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

   Depositor

   Daniel Bauer

   Article

   Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

   Inserts

   S. pyogenes sgRNA cassette
   Crimson Reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-a and hU6

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AICSDP-24:MYH10-mEGFP

   Plasmid

   #87428

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   MYH10 Homology Arms with mEGFP-linker (MYH10 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   May 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. DYRK1A gRNA (BRDN0001145031)

   Plasmid

   #76755

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DYRK1A

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DYRK1A (DYRK1A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. AICSDP-1:PXN-EGFP

   Plasmid

   #87420

   Purpose

   Homology arms and linker-EGFP sequence for C-terminus tagging of human PXN

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   PXN Homology Arms with linker-EGFP (PXN Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pYLsgRNA-OsU3m

   Plasmid

   #66193

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   OsU3m (SpeI site destroyed)

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSI-Target-AID-NG

   Plasmid

   #119861

   Purpose

   Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

   Depositor

   Osamu Nureki

   Article

   Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

   Insert

   nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Chr1q_Centromere-Targeting_gRNA

   Plasmid

   #195125

   Purpose

   gRNA targeting centromere-proximal location on Chromosome 1q in a third generation Cas9 backbone with GFP

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Chr1q gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AICSDP-34: TNNI1-mEGFP

   Plasmid

   #114411

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV_Nanog HMEJ donor

    Plasmid

    #97321

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Nanog HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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